纳氏试剂比色法测定污水中氨氮含量的影响因素探究  

Investigation on the Influence Factors to Determinate the Content of Ammonia Nitrogen in the Sewage with Nessler's Reagent Colorimetric Method

作　　者：张海涛[1] ZHANG Hai-tao(Offshore Oil Fudao Co.,Ltd.,Dongfang 572600,China)

机构地区：[1]海洋石油富岛有限公司,海南东方572600

出　　处：《化工管理》2021年第21期157-158,共2页Chemical Engineering Management

摘　　要：测定水中各种形态的氮化物含量有助于评价水体被污染和“自净”情况。纳氏试剂法由于操作简单、准确性高,被广泛用于测定水中氨氮的含量。本实验对污水中的氨氮进行测验时采用纳氏分光光度法,并制定了相关的标准曲线,其相关系数为0.99999。通过对标准曲线准确度及精密度进行检验,证明其具有较高的准确性(相对误差=0.00)及较好的精密度(RSD﹤5%)。通过纳氏试剂分光光度法测定了水中氨氮试验条件如反应显色时间、pH值、温度及样品放置时间对氨氮含量的影响。在本研究中,我们考察了两种不同氨氮的浓度在不同的实验条件下的实验结果。结果表明,显色时间应控制在10~30 min内;水样pH值应控制在6~8;反应温度应为25℃;每次测定的样品的显色时间不宜过长,时间越长,样品中氨氮的含量越高——应尽快测定水样吸光度,以获得准确可靠的实验结果。Determining the status of nitrogen compounds in water is helpful in assessing water pollution and‘automatic depuration’.Because of its simplicity,high accuracy,and extensive application,Nessler’s reagent method is adopted to determine ammonia nitrogen content in water.This spectrophotometric method was used to determine ammonia nitrogen in sewage,and the corresponding standard curve was formulated with a correlation coefficient of 0.99999.The standard curve was examined and a high accuracy(relative error=0.00)and good precision(RSD﹤5%)was confirmed.The experimental factors,i.e.,coloration time,pH value,temperature and settling time,that tend to influence the determination of ammonia nitrogen in water were tested using the Nessler’s reagent spectrophotometric method.In this research,two kinds of different ammonia nitrogen concentrations at varying experimental conditions were tested and the result indicated that the coloration time should be set at 10~30 min;pH value of water sample at 6~8;and reaction temperature at 25℃.The reaction time of sample was determined,it was unsuitably long and the longer the time,the higher the content of ammonia nitrogen.Therefore,the absorbance of water sample should be determined as fast as possible in an effort to obtain an accurate,reliable experimental result.

关 键 词：纳氏试剂 氨氮 影响因素 

分 类 号：X832[环境科学与工程—环境工程]