Hydrogen sulfide-linked persulfidation of ABI4 controls ABA responses through the transactivation of MAPKKK18 in Arabidopsis  

作　　者：Mingjian Zhou Jing Zhang Jie Shen Heng Zhou Didi Zhao Cecilia Gotor Luis CRomero Ling Fu Zongmin Li Jing Yang Wenbiao Shen Xingxing Yuan Yanjie Xie 

机构地区：[1]Laboratory Center of Life Sciences,College of Life Sciences,Nanjing Agricultural University,Nanjing 210095,PR China [2]Instituto de Bioquímica Vegetal y Fotosíntesis,Consejo Superior de Investigaciones Científicas y Universidad de Sevilla,Avenida Américo Vespucio 49,41092 Sevilla,Spain [3]State Key Laboratory of Proteomics,Beijing Proteome Research Center,National Center for Protein Sciences Beijing,Beijing Institute of Lifeomics,Beijing 102206,China [4]Institute of Industrial Crops,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China

出　　处：《Molecular Plant》2021年第6期921-936,共16页分子植物（英文版）

基　　金：supported by grants from the National Natural Science Foundation of China(31670255);the National Natural Science Foundation of China of Jiangsu Province(BK20200561,BK20200282,BK20161447);the National Science Fund for Outstanding Young Scholars(21922702);the China Postdoctoral Science Foundation(2019M661860);the Fundamental Research Funds for the Central Universities(KYZ201859);the European Regional Development Fund through the Agenda Estatal de Investigacion(grant no.PID2019-109785GB-IOO).

摘　　要：Hydrogen sulfide(H2S)is a signaling molecule that regulates plant hormone and stress responses.The phytohormone abscisic acid(ABA)plays an important role in plant adaptation to unfavorable environmental conditions and induces the persulfidation of L-CYSTEINE DESULFHYDRASE1(DES1)and the production of H2S in guard cells.However,it remains largely unclear how H2S and protein persulfidation participate in the relay of ABA signals.In this study,we discovered that ABSCISIC ACID INSENSITIVE 4(ABI4)acts downstream of DES1 in the control of ABA responses in Arabidopsis.ABI4 undergoes persulfidation at Cys250 that is triggered in a time-dependent manner by ABA,and loss of DES1 function impairs this process.Cys250 and its persulfidation are essential for ABI4 function in the regulation of plant responses to ABA and the H2S donor NaHS during germination,seedling establishment,and stomatal closure,which are abolished in the ABI4Cys250Ala mutated variant.Introduction of the ABI4Cys250Ala variant into the abi4 des1 mutant did not rescue its hyposensitivity to ABA.Cys250 is critical for the binding of ABI4 to its cognate motif in the promoter of Mitogen-Activated Protein Kinase Kinase Kinase 18(MAPKKK18),which propagates the MAPK signaling cascade induced by ABA.Furthermore,the DES1-mediated persulfidation of ABI4 enhances the transactivation activity of ABI4 toward MAPKKK18,and ABI4 can bind the DES1 promoter,forming a regulatory loop.Taken together,these findings advance our understanding of a post-translational regulatory mechanism and suggest that ABI4 functions as an integrator of ABA and MAPK signals through a process in which DES1-produced H2S persulfidates ABI4 at Cys250.

关 键 词：hydrogen sulfide persulfidation DES1 ABI4 MAPKXK18 transactivation ABA response 

分 类 号：Q94[生物学—植物学]