An early-morning gene network controlled by phytochromes and cryptochromes regulates photomorphogenesis pathways in Arabidopsis  

作　　者：Martin Balcerowicz Mahiar Mahjoub Duy Nguyen Hui Lan Dorothee Stoeckle Susana Conde Katja E.Jaeger Philip A.Wigge Daphne Ezer 

机构地区：[1]Sainsbury Laboratory,University of Cambridge,Cambridge,UK [2]Department of Statistics,University of Warwick,Coventry,UK [3]Alan Turing Institute,London,UK [4]Leibniz-Institute of Vegetable and Ornamental Crops(IGZ),14979 Großbeeren,Germany [5]Institute of Biochemistry and Biology,University of Potsdam,Potsdam,Germany [6]Department of Biology,University of York,York YO105DD,UK [7]Royal Prince Alfred Hospital,Central Clinical School,University of Sydney,Camperdown,NSW,Australia [8]Plant Systems Physiology,Institute for Water and Wetland Research,Radboud University,Nijmegen,the Netherlands [9]Zhejiang Normal University,Wucheng District,Jinhua,Zhejiang,China [10]Department of Plant and Microbial Biology,University of Zurich,Zurich,Switzerland

出　　处：《Molecular Plant》2021年第6期983-996,共14页分子植物（英文版）

基　　金：funded by an Alan Turing Institute Research fellowship under an EPSRC research grant(TU/A/000017);D.E.,EPSRC/BBSRC Innovation fellowships(EP/S001360/1 and EP/S001360/2);D.E.and S.C.,and an EMBO fellowship(ALTF 1418-2015)to M.B.

摘　　要：Light perception at dawn plays a key role in coordinating multiple molecular processes and in entraining the plant circadian clock.The Arabidopsis mutant lacking the main photoreceptors,however,still shows clock entrainment,indicating that the integration of light into the morning transcriptome is not well understood.In this study,we performed a high-resolution RNA-sequencing time-series experiment,sampling every 2 min beginning at dawn.In parallel experiments,we perturbed temperature,the circadian clock,photoreceptor signaling,and chloroplast-derived light signaling.We used these data to infer a gene network that describes the gene expression dynamics after light stimulus in the morning,and then validated key edges.By sampling time points at high density,we are able to identify three light-and temperature-sensitive bursts of transcription factor activity,one of which lasts for only about 8 min.Phytochrome and cryptochrome mutants cause a delay in the transcriptional bursts at dawn,and completely remove a burst of expression in key photomorphogenesis genes(HY5 and BBX family).Our complete network is available online(http://www-users.york.ac.uk/∼de656/dawnBurst/dawnBurst.html).Taken together,our results show that phytochrome and cryptochrome signaling is required for fine-tuning the dawn transcriptional response to light,but separate pathways can robustly activate much of the program in their absence.

关 键 词：BBX family CRYPTOCHROME dawn HY5 network MORNING PHYTOCHROME transcriptome 

分 类 号：Q94[生物学—植物学]