针刺“百会”透“曲鬓”穴对急性脑出血大鼠血肿及CD36、HO-1表达的影响  

Effect of Needling GV20-through-GB7 on Hematoma and Expressions of CD36 and HO-1 in Rats with Acute ICH

作　　者：陈秋欣[1,2] 孔莹[3] 于婷婷[1] 张瑜 刘鹏[1] 张鑫[1] 朱路文[3] CHEN Qiuxin;KONG Ying;YU Tingting;ZHANG Yu;LIU Peng;ZHANG Xin;ZHU LU Wen(The First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin 150040, China;Heilongjiang University of Chinese Medicine, Harbin 150040, China;The Second Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin 150001, China;Shenzhen Bao’an Hospital of Traditional Chinese Medicine (Group), Shenzhen 518133, China)

机构地区：[1]黑龙江中医药大学附属第一医院,黑龙江哈尔滨150040 [2]黑龙江中医药大学,黑龙江哈尔滨150040 [3]黑龙江中医药大学附属第二医院,黑龙江哈尔滨150001 [4]深圳市宝安中医院(集团),广东深圳518133

出　　处：《针灸临床杂志》2021年第8期59-63,共5页Journal of Clinical Acupuncture and Moxibustion

基　　金：国家自然科学基金,编号:82004442;黑龙江省自然科学基金,编号:H2018062。

摘　　要：目的:探讨针刺“百会”透“曲鬓”穴对急性脑出血大鼠血肿及其周围组织CD36、HO-1表达的影响,阐明针刺治疗脑出血的相关机制。方法:将108只Wistar雄性大鼠随机分为假手术组、模型组和针刺组,每组按1 d、3 d和7 d时间点再分3个亚组,每组12只。自体血注入法建立脑出血大鼠模型。采用针刺“百会”透“曲鬓”穴对模型大鼠进行干预。术后各时间点进行mNSS评分、血肿体积及脑水肿含量评估大鼠神经功能损伤以及脑损伤程度;Western-blot法检测血肿周围组织HO-1、CD36蛋白表达。结果:术后各时间点,与模型组比较,针刺组大鼠神经功能缺损评分降低(P<0.01);大鼠脑组织含水量降低(P<0.01);血肿周围脑组织HO-1、CD36蛋白表达升高(P<0.01)。术后3 d、7 d针刺组大鼠脑组织血肿体积缩小(P<0.05)。结论:针刺“百会”透“曲鬓”穴能促进脑出血大鼠血肿周围组织HO-1、CD36蛋白表达,减小血肿体积,减轻脑水含量,改善神经功能缺损。Objective:To explore the effect of needling Baihui(GV20)through Qubin(GB7)on hematoma and the expressions of CD36 and HO-1 in rats with acute intracerebral hemorrhage(ICH),thus to elaborate the related mechanism of acupuncture in treating ICH.Methods:108 Wistar male rats were randomly divided into the sham operation group,the model group and the acupuncture group,each group was subdivided into 3 subgroups according to 1d,3d and 7d,with 12 rats in each group.The ICH model was established by autologous blood injection in the rats.The acupuncture group was intervened by needling GV20 through GB7.mNSS score,hematoma volume and cerebral edema of each time point were used to assess the neurological function and cerebral damage.The protein expressions of CD36 and HO-1 around hematoma tissue were detected by Western-blot method.Results:At each postoperative time point,the scores of neurological deficits were decreased(P<0.01),the water content of the brain tissue was reduced(P<0.01),and the protein expressions of HO-1 and CD36 were increased in the acupuncture compared to those in the model group(P<0.01).The volume of hematoma in the brain tissue decreased after 3 d and 7 d of the operation in the acupuncture group(P<0.05).Conclusion:Needling GV20 through GB7 can promote the expressions of CD36 and HO-1,reduce the volume of hematoma,alleviate cerebral water content and improve the neurological function in the rats with ICH.

关 键 词：针刺 脑出血 神经功能 血红素氧化酶1 

分 类 号：R246.6[医药卫生—针灸推拿学]