血清miR-181b和miR-27a表达与颈动脉粥样硬化程度相关  

Correlation of serum miR-181b and miR-27a expression with the severity of carotid atherosclerosis

作　　者：张传西 张宇[1] 郭莎莎 厉菁[1] 袁义强[1] 王明杰 ZHANG Chuan-xi;ZHANG Yu;GUO Sha-sha;LI Jing;YUAN Yi-qiang;WANG Ming-jie(Department of Cardiology, Zhengzhou Seventh People's Hospital, Zhengzhou 450000, China)

机构地区：[1]郑州市第七人民医院心内科,河南郑州450000

出　　处：《基础医学与临床》2021年第8期1133-1138,共6页Basic and Clinical Medicine

基　　金：河南省医学科技攻关计划(2017T02096)。

摘　　要：目的分析血清微小RNA(miR)-181b、miR-27a表达与颈动脉粥样硬化(CAS)病变程度的关系。方法选取血脂异常患者102例,健康人100名为对照组,RT-qPCR检测血清miR-181b和miR-27a的表达。对比血脂异常患者与对照组血清miR-181b、miR-27a表达差异,分析血脂异常患者发生CAS的影响因素及血清miR-181b、miR-27a表达与CAS病变程度的相关性。结果血脂异常患者的血清miR-181b相对表达量低于对照组,而miR-27a相对表达量高于对照组(P<0.05);硬化组血清miR-181b相对表达量低于未硬化组,miR-27a相对表达量、hs-CRP、LDL-C水平、高血压占比高于未硬化组(P<0.05),Logistic回归分析显示以上指标均是血脂异常患者发生CAS的独立影响因素(P<0.05);ROC显示血清miR-181b、miR-27a相对表达量预测血脂异常患者发生CAS的灵敏度分别为84.75%、81.36%,特异度分别为76.74%、74.42%,准确度分别为81.37%、78.43%,AUC分别为0.791、0.750;Pearson相关性分析显示血清miR-181b相对表达量与Crouse积分呈负相关(P<0.05),miR-27a相对表达量与Crouse积分呈正相关(P<0.05)。结论血清miR-181b与miR-27a在血脂异常患者表达异常,且二者均是CAS发生的独立影响因素,其表达水平与病情严重程度有关。Objective To identify the relationship between expression of serum microRNA(miR)-181b,miR-27a and the severity of carotid atherosclerosis(CAS).Methods Totally 102 patients with dyslipidemia were selected and 100 healthy people as controls.RT-qPCR was used to detect the expression of serum miR-181b and miR-27a.The expression of miR-181b and miR-27a of patients with dyslipidemia was compared with that of the control group.The influencing factors of the occurrence of CAS in patients with dyslipidemia and the correlation between serum miR-181b,miR-27a levels and the degree of CAS lesions were investigated.Results The relative expression of miR-181b in dyslipidemia patients was lower than that of the control group,while the relative expression of miR-27a was higher than that of the control group(P<0.05).The expression of miR-181b in the cirrhosis group was lower than that in the non-cirrhotic group,and the relative expression of miR-27a,hs-CRP,LDL-C levels,andthe proportion of hypertension were higher than those in the non-cirrhotic group(P<0.05).Logistic regression analysis showed that the above indicators were independent factors influencing the occurrence of CAS in patients with dyslipidemia(P<0.05).ROC showed that the sensitivity of relative expression of miR-181b and miR-27a in serum was 84.75%and 81.36%in the predicting the occurrence of CAS in patients with dyslipidemia,the specificity was 76.74%and 74.42%,and the accuracy was 81.37%and 78.43%,respectively,AUC was 0.791,0.750 respectively.Pearson correlation analysis showed that the relative expression of serum miR-181b was negatively correlated with Crouse score(P<0.05),and the relative expression of miR-27a was positively correlated with Crouse score(P<0.05).Conclusions The expression of miR-181b and miR-27a in serum is abnormal in patients with dyslipidemia,and both of them are independent influencing factors of CAS,and their expression level is positively related to the severity of the disease.

关 键 词：颈动脉粥样硬化 血脂异常 微小RNA-181b 微小RNA-27a 

分 类 号：R543.4[医药卫生—心血管疾病]