翼茎羊耳菊提取物对脂多糖诱导的小鼠巨噬细胞RAW 264.7的抗炎作用  

The anti-inflammatory activity and mechanisms of Inula pterocaula extract in LPS-induced inflammatory responses in RAW 264.7 macrophages

作　　者：黄春丽 吴乐昊 于洋[1] 金慧子[1] 张岩 HUANG Chunli;WU Lehao;YU Yang;JIN Huizi;ZHANG Yan(School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China;School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China)

机构地区：[1]上海交通大学药学院,上海200240 [2]上海交通大学生命科学技术学院,上海200240

出　　处：《大连工业大学学报》2021年第4期253-259,共7页Journal of Dalian Polytechnic University

基　　金：国家自然科学基金面上项目(81973191);上海市自然科学基金项目(19ZR1428100).

摘　　要：研究了中药翼茎羊耳菊(IP)在脂多糖(LPS)诱导的RAW 264.7巨噬细胞中的抗炎活性和信号传导通路。通过Griess法测定一氧化碳(NO)水平;通过实时荧光定量PCR(RT-PCR)和酶联免疫吸附测定(ELISA)等方法检测药物对于LPS诱导产生的IL-6、IL-1β、TNF-α、iNOS在mRNA水平和蛋白质水平的影响;通过蛋白免疫印迹法(Western Blot)检测药物对于LPS所诱导的MAPK和NF-κB等炎症信号通路的作用。研究结果表明,翼茎羊耳菊乙酸乙酯组分IP(EA)可显著抑制LPS诱导的RAW 264.7细胞IL-6、IL-1β、TNF-α和iNOS的产生;可显著抑制LPS所诱导的MAPK和NF-κB通路的磷酸化。因此,翼茎羊耳菊通过抑制MAPK以及NF-κB通路发挥抗炎活性。The anti-inflammatory activity of Inula pterocaula(IP)extract and its mechanism of action in lipopolysaccharide(LPS)induced RAW 264.7 macrophage cells were explored.The production of NO was measured by Griess;the expression of IL-6,IL-1β,TNF-αand iNOS at mRNA and protein levels were detected by real time fluorescent quantitative PCR(RT-PCR)and enzyme-linked immunosorbent assay(ELISA);the MAPK and NF-κB signaling pathways were investigated by Western Blot.The results indicated that IP(EA)(the ethyl acetate of IP)significantly inhibited IL-6,IL-1β,TNF-αand iNOS in RAW 264.7 cells induced by LPS at both mRNA and protein levels.IP(EA)significantly suppressed the MAPK and NF-κB signaling pathways in LPS-stimulated RAW 264.7 cells.The result suggested that IP(EA)exerted anti-inflammatory activity by inhibiting MAPK and NF-κB signaling pathways.

关 键 词：翼茎羊耳菊 抗炎活性 丝裂原活化蛋白激酶 NF-κB 

分 类 号：R967[医药卫生—药理学]