Study on the Callus Induction and Petiole Proliferation of the Wild European Plum  

野生欧洲李叶柄愈伤组织诱导及增殖研究

作　　者：CHEN Xi GENG Wen-juan XIE Gang-gang XIE Jun 陈曦;耿文娟;颉刚刚;谢军(新疆农业大学林学与园艺学院,新疆乌鲁木齐830052;新疆农业科学院,新疆乌鲁木齐830052)

机构地区：[1]Xinjiang Agricultural University,College of Forestry and Horticulture,Urumqi 830052,PRC [2]Xinjiang Academy of Agricultural Sciences,Urumqi 830052,PRC

出　　处：《Agricultural Science & Technology》2021年第1期9-16,共8页农业科学与技术（英文版）

基　　金：国家自然科学基金资助项目(31560545);国家重点研发计划资助项目(2016YFC0501504);新疆维吾尔自治区园艺重点学科资助项目(2016-10758-3)。

摘　　要：[Objective]To establish the callus induction and proliferation system of the petiole of the wild European plum.[Methods]The petiole of the wild European plum was used as an explant.The effects of disinfection time,type and concentration of carbon source,dark treatment time,basic medium,growth regulator and sampling time on callus induction and petiole proliferation were studied.[Results]The best sampling time was from mid-May to mid-June.The lowest pollution rate was 3.33%when they were disinfected with 75%alcohol for 45 s and 0.1%mercuric chloride for 7 min.21 d of dark treatment was the best dark treatment time.The best formula for callus induction and proliferation was:B5+0.2 mg/L 6-BA+2 mg/L NAA+30 g/L sucrose+7 g/L agar.[Conclusion]A theoretical foundation was laid for the tissue culture and regeneration system of the wild European plum.[目的]为建立野生欧洲李叶柄愈伤组织诱导及增殖体系。[方法]以野生欧洲李叶柄为外植体,探究了消毒时间、碳源种类及其浓度、暗处理时间、基本培养基种类、生长调节剂种类及其配比和取材时间等因素对叶柄愈伤组织诱导及增殖的影响。[结果]最佳取材时间为5月中旬至6月中旬;以75%酒精消毒45 s+0.1%升汞消毒7 min,最低污染率为3.33%;暗处理21 d时,愈伤组织诱导及增殖最优配方为:B5+0.2 mg/L 6-BA+2 mg/L NAA+30 g/L蔗糖+7 g/L琼脂。[结论]为野生欧洲李组织培养再生体系奠定了理论基础。

关 键 词：Wild European plum PETIOLE Callus induction 

分 类 号：S662.3[农业科学—果树学]