Magnetic Micromanipulation for In Vivo Measurement of Stiffness Heterogeneity and Anisotropy in the Mouse Mandibular Arch  

作　　者：Min Zhu Kaiwen Zhang Hirotaka Tao Sevan Hopyan Yu Sun 

机构地区：[1]Department of Mechanical and Industrial Engineering,University of Toronto,Canada M5S 3G8 [2]Program in Developmental and Stem Cell Biology,Research Institute,The Hospital for Sick Children,Toronto,ON,Canada M5G 0A4 [3]Department of Molecular Genetics,University of Toronto,Canada M5S 1A8 [4]Division of Orthopaedic Surgery,The Hospital for Sick Children and University of Toronto,Canada M5G 1X8 [5]Institute of Biomaterials and Biomedical Engineering,University of Toronto,Canada M5S 3G9 [6]Department of Electrical and Computer Engineering,University of Toronto,Canada M5S 3G4

出　　处：《Research》2020年第1期1409-1419,共11页研究（英文）

基　　金：This work was funded by the Canadian Institutes of Health Research(CIHR);Natural Sciences and Engineering Research Council of Canada(NSERC),Ontario Research Funds,and Medicine by Design–New Ideas Program.

摘　　要：The mechanical properties of tissues are pivotal for morphogenesis and disease progression.Recent approaches have enabled measurements of the spatial distributions of viscoelastic properties among embryonic and pathological model systems and facilitated the generation of important hypotheses such as durotaxis and tissue-scale phase transition.There likely are many unexpected aspects of embryo biomechanics we have yet to discover which will change our views of mechanisms that govern development and disease.One area in the blind spot of even the most recent approaches to measuring tissue stiffness is the potentially anisotropic nature of that parameter.Here,we report a magnetic micromanipulation device that generates a uniform magnetic field gradient within a large workspace and permits measurement of the variation of tissue stiffness along three orthogonal axes.By applying the device to the organ-stage mouse embryo,we identify spatially heterogenous and directionally anisotropic stiffness within the mandibular arch.Those properties correspond to the domain of expression and the angular distribution of fibronectin and have potential implications for mechanisms that orient collective cell movements and shape tissues during development.Assessment of anisotropic properties extends the repertoire of current methods and will enable the generation and testing of hypotheses.

关 键 词：transition. stiffness anisotropic 

分 类 号：R318[医药卫生—生物医学工程]