红蜻稚虫对浙江省不同类型水体污染的抗逆性适应表现  

Resistance and adaptation of Crocothemis servilia larvae to different types of water pollution in Zhejiang Province

作　　者：俞玲园 季政权 胡耀文 徐风娇 王琪玮 魏莹 唐斌[1] 王世贵[1] YU Ling-Yuan;JI Zheng-Quan;HU Yao-Wen;Xu Feng-Jiao;WANG Qi-Wei;WEI Ying;TANG Bin;WANG Shi-Gui(School of Life and Environmental Sciences,Hangzhou Normal University,Hangzhou Key Laboratory of Animal Adaptation and Evolution,Hangzhou 310036,China)

机构地区：[1]杭州师范大学生命与环境科学学院,杭州市动物适应与进化重点实验室,杭州310036

出　　处：《环境昆虫学报》2021年第4期922-933,共12页Journal of Environmental Entomology

基　　金：杭州市科技重大专项计划(20190101A01);国家级大学生创新创业训练计划项目(201910346059);浙江省大学生科技创新活动计划暨新苗人才计划(2018R413079)。

摘　　要：在杭州、温州、宁波、台州和绍兴等地水域5个采样点采集到169头蜻蜓稚虫,分属于7科。对采集地的水质进行理化指标(水温、压强、电导率、氧化还原电位、pH、溶解氧、总氮、总磷、高锰酸盐指数、叶绿素a、透明度)和重金属含量(Cu^(2+)、Zn^(2+)、Cd^(2+)、Pb^(2+)、Cr^(6+)和总铁等)检测,并对采集到的红蜻稚虫Crocothemis servilia检测其体内的SOD和CAT酶活力,以及HSP70、HSP90和CYP303a1等抗逆性相关基因的表达量。结果表明:生活在不同水体中的红蜻稚虫,其体内的SOD和CAT酶活力主要与Cr^(6+)浓度正相关,可在一定程度上反映出某一水体的Cr^(6+)污染状况;HSP70的表达量与水体TN、TP和Pb^(2+)浓度间呈正相关,同时HSP90的表达与水体TP和COD_(Mn)浓度正相关,可反映水体富营养化的主要污染物类型。红蜻稚虫CYP303a1的表达水平与水体中Cr^(6+)含量呈负相关,而与TP和Cd^(2+)浓度呈正相关。推测在工业废水污染下,红蜻稚虫体内的SOD、CAT和CYP303a1能帮助红蜻稚虫适应在Cr^(6+)浓度较高的水体中生活,而不同片段长度的热激蛋白HSP可能与抵抗不同类型的富营养化污染有关系。实验结果为进一步探究蜻蜓稚虫的抗逆性适应机制提供了基础数据。Dragonfly larvae were collected from 5 collection sites such as Hangzhou,Wenzhou,Ningbo,Taizhou and Shaoxing,169 dragonfly larvae were identified belonging to 7 families.Physical and chemical indexes(water temperature,pressure,conductivity,redox potential,pH,dissolved oxygen,total nitrogen,total phosphorus,permanganate index,chlorophyll a,transparency)and heavy metal content(Cu^(2+),Zn^(2+),Cd^(2+),Pb^(2+),Cr^(6+)and total iron,etc.)were tested on the water quality of the collection sites,and the enzyme activity of SOD and CAT and the expressions of HSP70,HSP90 and CYP303a1 of the collected Crocothemis servilia were tested.The results showed that the enzyme activity of SOD and CAT in C.servilia larvae living in different water bodies was positively correlated with the concentration of Cr^(6+),which could reflect the pollution status of Cr^(6+)in a certain water body to some extent.The expression of HSP70 was positively correlated with the concentration of TN,TP and Pb^(2+),and the expression of HSP90 was positively related to the concentration of TP and COD_(Mn),which can reflect the main pollutants of eutrophication in water body.The expression of CYP303a1 was negatively correlated with Cr^(6+),but positively correlated with concentration of TP and Cd^(2+).It was speculated that SOD,CAT and CYP303a1 in C.servilia could help them to adapt to live with high Cr^(6+)concentration under industrial water pollution,and the heat shock proteins of different fragment lengths might be related to resistance to different types of eutrophication pollution.The experimental results may provide basic data for further exploration of the stress resistance and adaptation mechanism of dragonfly larvae.

关 键 词：红蜻稚虫 水体污染 重金属 SOD CAT HSP CYP303a1 抗逆性表现 

分 类 号：Q968.1[生物学—昆虫学] S433[农业科学—农业昆虫与害虫防治]