广聚萤叶甲取食对豚草挥发物组分的影响  

Effects of herbivory by the leaf beetle Ophraella communa on volatile compounds of the invasive common ragweed Ambrosia artemisiifolia

作　　者：崔少伟 马超 张燕 白强 陈红松[4] 臧连生 周忠实[2] CUI Shao-Wei;MA Chao;ZHANG Yan;BAI Qiang;CHEN Hong-Song;ZANG Lian-Sheng;ZHOU Zhong-Shi(College of Plant Protection,Jilin Agricultural University,Changchun 130118,China;State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China;College of Plant Protection,Yunnan Agricultural University,Kunming 650201,China;Guangxi Key Laboratory for Biology of Crop Diseases and Insect Pests,Institute of Plant Protection,Guangxi Academy of Agricultural Sciences,Nanning 530007,China)

机构地区：[1]吉林农业大学植物保护学院,长春130118 [2]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193 [3]云南农业大学植物保护学院,昆明650201 [4]广西壮族自治区农业科学院植物保护研究所,广西农作物病虫害生物学重点实验室,南宁530007

出　　处：《环境昆虫学报》2021年第4期1023-1033,共11页Journal of Environmental Entomology

基　　金：国家自然科学基金(31672089)。

摘　　要：植物在遭受植食性昆虫为害后,会系统性地释放虫害诱导挥发物(HIPVs)。为探明广聚萤叶甲取食对豚草挥发物组分的影响,本研究采用动态顶空吸附法和气相色谱-质谱联用仪(GC-MS),收集并分析了豚草在受机械损伤和受广聚萤叶甲取食损伤前后挥发物组分及相对含量的变化。结果表明,豚草健康植株与损伤植株的挥发物组分和相对含量具有明显的差异。与健康植株相比,受机械损伤后的豚草挥发物中新出现了乙酸芳樟酯、桉树醇、十二醛、γ-木罗烯、植物醇、β-波旁烯,这些化合物在受损后立即释放,表明它们是组成型豚草挥发性有机化合物(VOCs);受取食损伤后,挥发物中大根香叶烯D的相对含量显著增加,表明大根香叶烯D为诱发型VOCs;而较于健康植株和机械损伤植株,取食损伤后的豚草VOCs中增加了桧烯、辛醛、β-罗勒烯、香茅醇,表明这些化合物为HIPVs。此外,机械损伤与取食损伤对豚草VOCs组分的影响有所不同。受机械损伤后,挥发物的种类减少后(损伤后第3天)很快又恢复到健康水平,而受取食损伤后,VOCs的种类逐渐减少(损伤后3 d内)。受损后不同时期挥发物的种类和相对含量亦有显著差异。受损后的早期(1 d),释放的多为一些豚草绿叶性物质,而在后期有一些诱导物释放。本研究结果为利用豚草VOCs和HIPVs组分提高广聚萤叶甲对豚草的防控效果奠定了重要基础。Plants can systematically release herbivore-induced plant volatiles(HIPVs)after being damaged by herbivorous insects.To understand the effects of herbivory by the leaf beetle Ophraella communa on volatile compounds of the invasive common ragweed Ambrosia artemisiifolia,the changes of volatile compounds and relative contents of common ragweed before and after mechanical and feeding damages by the beetle were collected and analyzed by dynamic headspace adsorption and gas chromatography-mass spectrometry(GC-MS).The results showed that there were significant differences in the volatile compounds and relative contents between healthy and damaged plants.Compared with healthy plants,mechanically damaged plants instantly released Linalyl acetate,Eucalypto,Dodecanal,Phytol,γ-Muurolen,phytol andβ-Bourbonene,suggesting that these compounds were constitutive volatile organic compounds(VOCs).After being damaged by feeding,the plant increased relative contents of Germacrene D,suggesting its inducing VOCs.Compared with healthy plants and mechanically damaged plants,the feeding damaged plant released unique chemical including sabinene,octanal,β-Ocimene and Citronellol,suggesting that they were induced by the feeding treatment,these volatiles were HIVPs.Moreover,the effects of mechanical damage and feeding damage had differential effects on ragweed,the former causing a rapid decrease in volatiles released post damage back to the level of healthy plants at 3 days while the latter effecting a gradual decrease in volatiles.In addition,temporal pattern of volatile release differed between mechanical and feeding damage,with the volatiles of green leaves being released at an early stage after mechanical damage while at a late stage after feeding damage.The results from this study suggested that the common ragweed plants could be induced to release volatiles in response to feeding damage by specialist herbivore O.communa.

关 键 词：豚草 广聚萤叶甲 机械损伤 挥发物 物质鉴定 

分 类 号：Q968.1[生物学—昆虫学] S476[农业科学—农业昆虫与害虫防治]