阿尔泰蝠蛾幼虫血细胞形态学研究  

Morphology of the hemocytes of Hepialus altaicola Wang(Lepidoptera:Hepialidae)

作　　者：孙涛 张婷婷[2] 王岩[1,3,4] SUN Tao;ZHANG Ting-Ting;WANG Yan(Medical College of Shihezi University,Shihezi 832000,Xinjiang Uygur Autonomous Region,China;The Third Affiliated Hospital of Shihezi University(Shihezi People s Hospital),Shihezi 832000,Xinjiang Uygur Autonomous Region,China;College of Animal Science and Technology,Shihezi University 832000,Xinjiang Uygur Autonomous Region,China;Animal Husbandry Post-doctoral of Shihezi University,Shihezi 832000,Xinjiang Uygur Autonomous Region,China)

机构地区：[1]石河子大学医学院,新疆维吾尔自治区石河子832000 [2]八师石河子总医院(石河子大学第三附属医院,石河子市人民医院),新疆维吾尔自治区石河子832000 [3]石河子大学动物科技学院,新疆维吾尔自治区石河子832000 [4]石河子大学畜牧学博士后流动站,新疆维吾尔自治区石河子832000

出　　处：《环境昆虫学报》2021年第4期1061-1069,共9页Journal of Environmental Entomology

基　　金：国家自然科学基金(32060125,81560614);中国博士后科学基金(2016M602907)。

摘　　要：本研究采用Wright-Giemsa染色方法,利用光学显微镜观察阿尔泰蝠蛾Hepialus altaicola Wang幼虫血细胞。根据鳞翅目昆虫血细胞形态特征对阿尔泰蝠蛾幼虫血细胞进行鉴定,计数并计算各类血细胞所占的比例。发现阿尔泰蝠蛾幼虫血淋巴中存在原血胞、浆血胞、粒血胞、类绛色细胞、珠血胞和囊血胞6种细胞类型,所占比例分别为3.7%,29.9%,55.4%,2.3%,5.8%和2.9%。阿尔泰蝠蛾幼虫血细胞组成及其形态与其他鳞翅目昆虫基本一致,但是与其它种属相比,同种类型的血细胞大小和占比略有不同。粒血胞和浆血胞是阿尔泰蝠蛾幼虫血淋巴中的主要类型,其中粒血胞占比最高。该研究为深入研究阿尔泰蝠蛾血细胞形态和功能提供理论依据和基础数据。Hemocytes of Hepialus altaicola Wang(Lepidoptera:Hepialidae)larvae were stained with Wright-Giemsa and observed under an optical microscope.The hemocytes were identify of H.altaicola larvae according to the morphological characteristics of lepidopteran insects and calculate the proportion of various types of hemocytes.The results revealed that there were prohemocytes,plasmatocytes,granulocytes,oenocytoids,spherulocytes and cystocytes in H.altaicola,accounting for 3.7%,29.9%,55.4%,2.3%,5.8%and 2.9%,respectively.The composition and morphology of hemocytes of H.altaicola larvae were basically the same as those of other lepidopteran insects.But compared with other species,the size and proportion of hemocytes of the same type were slightly different.Granulocytes and plasmatocytes were the main types of hemolymph in H.altaicola larvae,among which the granulocytes had the highest proportion.This study provided theoretical basis and basic data for in-depth study of the morphology and function of H.altaicola hemocytes.

关 键 词：阿尔泰蝠蛾 血细胞 血淋巴 Wright-Giemsa染色 

分 类 号：Q965[生物学—昆虫学] S89[农业科学—特种经济动物饲养]