基于菲并咪唑的ON-OFF-ON双比色荧光探针及细胞成像  

ON-OFF-ON Double Colorimetric and Fluorescent Probes Based on Phenanthro[9,10-d]imidazole Derivatives and Their Living Cells Imaging

作　　者：李安然 赵冰[1,2] 阚伟[1,2] 宋天舒[1] 孔祥东 卜凡强[1] 孙立[1,2] 殷广明[1] 王丽艳[1] LI Anran;ZHAO Bing;KAN Wei;SONG Tianshu;KONG Xiangdong;BU Fanqiang;SUN Li;YIN Guangming;WANG Liyan(College of Chemistry and Chemical Engineering,Qiqihar University,Qiqihar University,Qiqihar 161006,China;Heilongjiang Provincial Key Laboratory of Surface Active Agent and Auxiliary,Qiqihar University,Qiqihar University,Qiqihar 161006,China)

机构地区：[1]齐齐哈尔大学化学与化学工程学院,齐齐哈尔161006 [2]齐齐哈尔大学黑龙江省表面活性剂与工业助剂重点实验室,齐齐哈尔161006

出　　处：《高等学校化学学报》2021年第8期2403-2411,共9页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:21978140);黑龙江省科技厅留学归国人员科学基金项目(批准号:LC2017004);黑龙江省省属高校基本科研业务费(批准号:YSTSXK201853,YSTSXK201859);黑龙江省人力资源和社会保障厅省级领军人才梯队后备带头人项目;齐齐哈尔大学研究生创新科研项目(批准号:YJSCX2020005和YJSCX2019037)资助。

摘　　要：设计合成了对称型菲并咪唑荧光探针PIP-ph-PIP,并对其结构进行了表征和确认.随着探针溶液中水体积分数的增加,探针的聚集程度逐渐增加,荧光强度先增强后猝灭.荧光光谱测试结果表明,在水相体系中探针PIP-ph-PIP能以ON-OFF-ON的方式分别对Ag^(+)和SCN~-,Cu^(2+)和PO_(4)^(3-)进行连续识别,且连续识别效果可通过裸眼比色观察,其中对Ag^(+)识别具有超低检出限(6.1 nmol/L).结果表明,探针PIP-ph-PIP可应用于活体He La细胞中Ag^(+)和Cu^(2+)的定性分析及实际水样中Ag^(+)和Cu^(2+)的定量检测.The symmetric fluorescent probe PIP-ph-PIP based on phenanthro[9,10-d]imidazole was designed and synthesized,and its structure was characterized and confirmed.As the increasing amount of water in the solution of PIP-ph-PIP,the fluorescence intensity of PIP-ph-PIP first increased and then quenched with the gradual aggregation.The results of the fluorescence spectra indicated that the probe PIP-ph-PIP could selectively and successively recognize Ag^(+)and SCN^(-),Cu^(2+)and PO_(4)^(3-),respectively,with an ON-OFF-ON mode in the aqueous system.Moreover,the continuous effect can be observed by the colorimetric naked eye.The probe PIP-ph-PIP offered a relatively low detection limits of 6.1 nmol/L for Ag^(+)ions.The probe PIP-ph-PIP was successfully applied to detect Ag^(+)and Cu^(2+)in living human cancer cells(He La cells)and quantitively analyze of Ag^(+)and Cu2+in actual water samples.

关 键 词：水相 菲并咪唑 比色 荧光探针 细胞成像 

分 类 号：O657.3[理学—分析化学]