冠心平含药血清对氧化低密度脂蛋白诱导的RAW264.7巨噬细胞泡沫化的影响  

Effect of Serum Containing Guanxinping on the Foaming of RAW264.7 Macrophages Induced by Oxidized Low-density Lipoprotein

作　　者：周冠进 严飞 孙玉婷 陈韦凯 掌琳惠 袁冬平[2] 刘福明 ZHOU Guan-jin;YAN Fei;SUN Yu-ting;CHEN Wei-kai;ZHANG Lin-hui;YUAN Dong-ping;LIU Fu-ming(Lianshui Hospital of Traditional Chinese Medicine, Jiangsu Huai'an 223400, China;Nanjing University of Chinese Medicine, Jiangsu Nanjing 210023, China;The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Nanjing 210023, China)

机构地区：[1]涟水县中医院,江苏淮安223400 [2]南京中医药大学,江苏南京210023 [3]南京中医药大学附属医院,江苏南京210023

出　　处：《安徽中医药大学学报》2021年第4期79-82,共4页Journal of Anhui University of Chinese Medicine

基　　金：江苏省重点研发计划项目(BE2020683);江苏省中医药科技发展计划重点项目(ZD201906);江苏省中医院创新发展基金项目(Y2019CX20)。

摘　　要：目的观察冠心平含药血清对氧化低密度脂蛋白(oxidized low-density lipoprotein,ox-LDL)诱导的RAW264.7巨噬细胞泡沫化的调控作用。方法将RAW264.7巨噬细胞分为对照组,模型组,冠心平含药血清低、中、高剂量组,以80μg/mL ox-LDL刺激24 h,诱导巨噬细胞泡沫化模型,采用不同浓度冠心平含药血清进行干预。油红O染色观察巨噬细胞内脂滴形成;ELISA法检测巨噬细胞内游离胆固醇(free cholesterol,FC)与胆固醇酯(cholesterol esterase,CE)的表达水平;实时荧光定量PCR法检测巨噬细胞清道夫受体A(scavenger receptor-A,SR-A)和CD36 mRNA表达水平。结果冠心平中、高剂量能够显著减少巨噬细胞泡沫化;低剂量显著增加巨噬细胞FC水平(P<0.05),高剂量显著降低巨噬细胞CE水平(P<0.05);冠心平高剂量显著增加SR-A mRNA表达水平(P<0.05),低、中、高剂量均显著增加CD36 mRNA表达水平(P<0.05)。结论冠心平能够抑制巨噬细胞的泡沫化,这或许是冠心平治疗动脉粥样硬化的潜在机制之一。Objective To investigate the regulatory effect of serum containing Guanxinping on the foaming of RAW264.7 macrophages induced by oxidized low-density lipoprotein(ox-LDL).Methods RAW264.7 macrophages were divided into control group,model group,and low-,middle-,and high-dose serum containing Guanxinping groups.RAW264.7 macrophages were stimulated by 80μg/mL ox-LDL for 24 hours to establish a model of macrophage foaming,and different concentrations of serum containing Guanxinping were used for intervention.Oil red O staining was used to observe the formation of lipid droplets in macrophages;ELISA was used to measure the expression of free cholesterol(FC)and cholesterol esterase(CE)in macrophages;quantitative real-time PCR was used to measure the mRNA expression of SR-A and CD36 in macrophages.Results Middle-and high-dose Guanxinping significantly reduced the foaming of macrophages.Low-dose Guanxinping significantly increased the level of FC in macrophages(P<0.05),and high-dose Guanxinping significantly reduced the level of CE in macrophages(P<0.05).High-dose Guanxinping significantly increased the mRNA expression level of SR-A(P<0.05),and low-,middle-,and high-dose Guanxinping significantly increased the mRNA expression level of CD36(P<0.05).Conclusion Guanxinping can inhibit the foaming of macrophages,which may be one of the potential mechanisms of Guanxinping in the treatment of atherosclerosis.

关 键 词：冠心平 动脉粥样硬化 巨噬细胞 泡沫化 

分 类 号：R541.4[医药卫生—心血管疾病]