唾液阿片类毒品的毛细管电泳/质谱联用分析方法研究  

Determination of opiate alkaloids in saliva using capillary electrophoresis-mass spectrometric

作　　者：王燕燕 宋蕾[2] 张瑛 张文芳 于子辉 孟品佳[3] 张敏[4] Wang Yanyan;Song Lei;Zhang Ying;Zhang Wenfang;Yu Zihui;Meng Pinjia;Zhang Min(Key Laboratory of Forensic Toxicology,Ministry of Public Security,People’s Republic of China,Beijing,100192;Beijing Police College,Beijing 102202;People's public security university of China,Beijing 100038;Dong Cheng suboffice,Beijing Public Security Bureau,Beijing 100010)

机构地区：[1]法庭毒物分析公安部重点实验室,北京100192 [2]北京警察学院,北京102202 [3]中国人民公安大学,北京100038 [4]北京市公安局东城分局,北京100010

出　　处：《现代科学仪器》2021年第4期66-71,共6页Modern Scientific Instruments

摘　　要：本文在对电泳分离条件和质谱检测条件进行考查的基础上,建立了阿片类毒品的毛细管电泳/质谱联用(CE/MS)分析方法。采用含15%甲醇浓度为80mmol/L乙酸铵-乙酸(pH=5.1)缓冲溶液作为背景缓冲体系,含15mmol/L乙酸铵和0.25%乙酸的60%甲醇水溶液作为鞘液,0.5psi压力下进样10s,分离电压25kV。在优化条件下,吗啡、6-单乙酰吗啡、可待因和海洛因4种毒品的唾液添加样本稳定性(RSD,n=5)均≤5%,检测限为10~50ng/mL。该方法稳定性好,灵敏度高,可以用于唾液中4种阿片类毒品的筛查。This paper describes the determination of opiate alkaloids(morphine,codeine,oripavine and thebaine)usingcapillary coupled to time-of-flight mass spectrometry.Parameters affecting separation efficiency and ESI-MS ionize conditions were investigated and optimized,including status of buffer solution and sheath liquid composition,flow rate etc.80 mmol/L sodium acetate-acetate acid buffer(pH 5.1)containing 15%methanol(v/v)was used as the background electrolyte for CE separation.A mixture of water and methanol(60%)containing ammonium acetate(15mmol/L)and acetate acid(0.25%)as sheath liquid.The samples were introduced for 10s under a pressure of 0.5 psi.The method showed the LODs in the range of 10~50ng/mL,while providing good separation efficiency and reproducibility(RSD≤5%,n=5).The feasibility of this method was demonstrated by analyzing saliva spiked opiates.

关 键 词：阿片类毒品 毛细管电泳/质谱联用 唾液 

分 类 号：O65[理学—分析化学]