白介素-13、白介素-1受体拮抗剂和环氧合酶-2在炎症性肠病患者血清中的表达及临床意义  

Expressions and clinical significance of interleukin-13,interleukin-1 receptor antagonist and cyclooxygenase-2 in the serum of patients with inflammatory bowel disease

作　　者：张现明 杨锦平 ZHANG Xianming;YANG Jinping(Department of Blood Transfusion,Bao'an Central Hospital of Shenzhen City,Guangdong,Shenzhen 518102,China)

机构地区：[1]深圳市宝安区中心医院输血科,广东深圳518102

出　　处：《中国医药科学》2021年第13期137-141,共5页China Medicine And Pharmacy

摘　　要：目的探究白介素(IL)-13、白介素-1受体拮抗剂(IL-1Ra)和环氧合酶-2(COX-2)在炎症性肠病(IBD)患者血清中的表达及临床意义。方法选择2018年3月至2019年3月在深圳市宝安区中心医院治疗的124例均处于活动期的IBD患者为试验组,其中克罗恩病(CD)60例为A组和溃疡性结肠炎(UC)64例为B组,CD参照克罗恩病简化内镜(SES-CD)评分标准,将A组分别为18例轻度活动期为组A1、26例中度活动期为A2组和16例重度活动期为A3组;UC参照改良Truelove和Witts疾病严重程度评分标准,把B组分别为21例轻度活动期为组B1、25例中度活动期为B2组和18例重度活动期为B3组;同期选健康组100例为对照组;均采用酶联免疫吸附测定法(ELISA)法检测各组受检者血清IL-13、IL-1Ra和COX-2水平,分析血清IL-13、IL-1Ra和COX-2水平与疾病活动度。结果实验组的IL-13、IL-1Ra和COX-2表达显著高于对照组,差异有统计学意义(P<0.05);血清IL-13、IL-1Ra和COX-2联合检测对于IBD的诊断效能较单独检测高,B组的IL-13、IL-1Ra和COX-2表达跟A组比较,差异有统计学意义(P<0.05);B组中B1、B2和B3患者血清IL-13、IL-1Ra和COX-2水平比较,差异有统计学意义(P<0.05),IL-13、IL-1Ra和COX-2表达水平与UC疾病严重程度呈正相关(R^(2)分别为0.78、0.85和0.82,P<0.05);A组中A1、A2和A3患者血清IL-13、IL-1Ra和COX-2水平比较,差异有统计学意义(P<0.05),IL-13、IL-1Ra和COX-2表达水平与CD疾病严重程度呈正相关(R^(2)分别为0.76、0.84和0.81,P<0.05)。结论IBD患者血清IL-13、IL-1Ra和COX-2水平明显升高,且与UC和CD活动性有关。IL-13、IL-1Ra和COX-2的表达和UC及CD的活性密切相关,可作为判断UC及CD活性的指标。Objective To probe into the expressions and clinical significance of interleukin-13(IL-13),interleukin-1 receptor antagonist(IL-1 Ra)and cyclooxygenase-2(COX-2)in the serum of patients with inflammatory bowel disease(IBD).Methods A total of 124 patients with IBD who were all in active stage treated in Bao’an Central Hospital of Shenzhen City from March 2018 to March 2019 were selected as the test group,with 60 cases of Crohn’s disease(CD)in the group A and 64 cases of ulcerative colitis(UC)in the group B.In terms of CD,with reference to the Simplified Endoscopic Score for Crohn’s Disease(SES-CD),the group A was divided into group A1,A2 and A3,with 18 cases at the mild active stage as group A1,26 cases at the moderate active stage as group A2 and 16 cases at the severe active stage as group A3.In terms of UC,with reference to the modified Truelove and Witts disease severity scoring criteria,the group B was divided into group B1,B2 and B3,with 21 cases at the mild active stage as group B1,25 cases at the moderate active stage as group B2 and 18 cases at the severe active stage as group B3.100 cases of healthy people were selected as the control group at the same period.The levels of serum IL-13,IL-1 Ra and COX-2 in each group were measured by the enzyme-linked immunosorbent assay(ELISA).The correlation between the levels of serum IL-13,IL-1 Ra,COX-2 and the disease activity were analyzed.Results The expressions of IL-13,IL-1 Ra and COX-2 in the test group were significantly higher than those in the control group,with statistically significant differences(P<0.05).The combined measurement of serum IL-13,IL-1 Ra and COX-2 had higher diagnostic efficacy for IBD than measured individually,and there were statistically significant differences between group B and group A in the expressions of IL-13,IL-1 Ra and COX-2(P<0.05).There were statistically significant differences between group B1,B2 and B3 in the levels of serum IL-13,IL-1 Ra and COX-2(P<0.05).IL-13,IL-1 Ra and COX-2 expression levels were positively correla

关 键 词：白介素-13 白介素-1受体拮抗剂 环氧合酶-2 炎症性肠病 溃疡性结肠炎 克罗恩病 

分 类 号：R259[医药卫生—中西医结合]