低氘水联合富血小板血浆对糖尿病大鼠胰岛细胞的保护作用及TGF-β_(1)表达的影响  

Effects of Deuterium-depleted Water Combined with Platelet-rich Plasma on Islet Cell Protection and TGF-β_(1) Expression in Diabetic Rats

作　　者：赵超然 王湘琦 熊爱兵 ZHAO Chao-ran;WANG Xiang-qi;XIONG Ai-bing(Department of Plastic and Bum Surgery,Affiliated Hospital of Southwest Medical University,National Key Clinical Construction Specialty,Luzhou 646000,Sichuan,China)

机构地区：[1]西南医科大学附属医院整形烧伤外科国家临床重点建设专科,四川泸州646000

出　　处：《中国美容医学》2021年第6期101-106,共6页Chinese Journal of Aesthetic Medicine

摘　　要：目的:观察低氘水(Deuterium-depleted water,DDW)联合富血小板血浆(Platelet-rich plasma,PRP)对糖尿病大鼠胰岛细胞保护作用及TGF-β_(1)表达的影响,初步探讨其对保护糖尿病大鼠胰岛细胞、促进糖尿病溃疡愈合的可能机制。方法:通过高糖高脂饲料喂养配合腹腔注射链脲菌素(STZ)+背部皮肤全层切除建立糖尿病大鼠溃疡模型。成模后按随机数字法分为糖尿病模型组、低氘水组(DDW)、富血小板血浆组(PRP)和低氘水联合富血小板血浆组(DDW+PRP)。空白对照组采用腹腔注射柠檬酸-柠檬酸钠缓冲液+背部皮肤全层切除建模。各组大鼠分别于治疗后3d、7d、14d检测随机血糖,创面愈合率。胰腺组织HE染色后观察其病理学改变,酶联免疫吸附法检测创面组织TGF-β_(1)表达情况。结果:DDW组、DDW+PRP组干预14d后随机血糖较干预前降低,差异具有统计学意义(P<0.05)。DDW组、PRP组、DDW+PRP组在干预7d、14d后创面愈合率高于糖尿病模型组,差异具有统计学意义(P<0.05);DDW+PRP组在干预14d时创面愈合率高于DDW组、PRP组,差异具有统计学意义(P<0.05);且与空白对照组相比,差异无统计学意义(P>0.05)。胰岛组织学观察随干预时间延长,DDW组、DDW+PRP组较前相比,胰岛细胞在形态、数量、排列方式、染色颗粒分布情况均有明显改观。DDW+PRP组干预后7d、14d后TGF-β_(1)含量高于DDW组、PRP组,差异具有统计学意义(P<0.05);而DDW组、PRP组相比差异无统计学意义(P>0.05)。结论:低氘水联合富血小板血浆对2型糖尿病大鼠溃疡创面的愈合有显著促进作用,且低氘水对2型糖尿病大鼠胰岛细胞具有一定保护和修复作用。促进创面愈合机制可能与降低糖尿病大鼠随机血糖,改善胰岛细胞功能,提高创面组织中TGF-β_(1)含量有关。Objective To observe the effect of deuterium-depleted water(DDW)combined with platelet-rich plasma(PRP)on the islet cell protection and TGF-β_(1)expression in diabetic rats,and explore its possible mechanism to protect islet cells of diabetic rats and promote the healing of diabetic ulcer.Methods The ulcer model of diabetic rats were established by feeding high-sugar and high-fat feed combined with intraperitoneal injection of streptozotocin(STZ)+full-thickness resection of back skin.After modeling,they were divided into diabetes model group,deuterium-depleted water group(DDW),platelet-rich plasma group(PRP)and deuterium-depleted water combined with platelet-rich plasma group(DDW+PRP)according to random numbers.The blank control group was modeled by intraperitoneal injection of citric acid-sodium citrate buffer+full-thickness resection of the back skin.Rats in each group were tested for random blood glucose and wound healing rate 3d,7d,14d after treatment.The pathological changes were observed after HE staining of pancreatic tissue.Enzyme-linked immunosorbent assay was used to detect the expression of TGF-β_(1)in wound tissue.The pathological changes were observed.Enzyme-linked immunosorbent assay was used to detect the expression of TGF-β_(1)in wound tissue.Results The random blood glucose in the DDW group and DDW+PRP group after 14 days of intervention was lower than before,the difference was statistically significant(P<0.05).The wound healing rate of the DDW group,PRP group,and DDW+PRP group was higher than the diabetes model group after 7d and 14d intervention(P<0.05).The wound healing rate of the DDW+PRP group was higher than DDW group and PRP group(P<0.05),and compared with the blank control group,the difference was not statistically significant(P>0.05).With the extension of the intervention time,compared with the previous groups,the islet cells in the DDW group and DDW+PRP group were significantly improved in their morphology,number,arrangement,and distribution of stained particles.The content of TGF-β

关 键 词：低氘水 富血小板血浆 糖尿病 胰岛细胞 溃疡创面 转化生长因子β_(1) 

分 类 号：R587.1[医药卫生—内分泌]