Multiple olfactory pathways contribute to the lure process of Caenorhabditis elegans by pathogenic bacteria  

作　　者：Man Zhu Yao Chen Ninghui Zhao Hua Bai Keqin Zhang Xiaowei Huang 

机构地区：[1]State Key Lab for Conservation and Utilization of Bio-Resources,and College of Life Science,Yunnan University,Kunming 650091,China [2]Neurosurgery of the Second Hospital Affiliated with Kunming Medical University,Kunming 650101,China [3]College of Public Health,Kunming Medical University,Kunming 650500,China

出　　处：《Science China(Life Sciences)》2021年第8期1346-1354,共9页中国科学（生命科学英文版）

基　　金：supported by the Department of Science and Technology of Yunnan Province (2019FA046);the National Natural Science Foundation of China (32060632 and 31370162)

摘　　要：Chemosensation is indispensable for the survival of Caenorhabditis elegans to discriminate food and pathogenic bacteria in their living environment. Food-like odors emitted by the pathogen Bacillus nematocida B16 for trapping its hosts and an olfactory signaling pathway responsible to sense the attractant 2-heptanone were identified in our previous study. Here, we further explore how the worms recognize the attractive molecules indole and 2-ethyl hexanol, which have different chemical properties and modest nematode-luring ability. We show that the chemotaxis toward indole and 2-ethyl hexanol requires the G protein-coupled receptors encoded by str-193 on AWC and str-7 on AWA. In a further genetic screen for downstream effectors in olfactory signaling cascades, the Gα subunit GSA-1, guanylyl cyclase ODR-1 and DAF-11 and the c GMP-gated channel TAX-2/TAX-4 were found to be necessary for indole sensation, whereas the TRPV channels OSM-9/OCR-2 and the PLC pathway activated by GPA-6 are responsible for the detection of 2-ethyl hexanol. Altogether, our current work further clarifies the distinct olfactory signaling pathways through which C. elegans senses different chemicals and is lured by B. nematocida B16, improving our comprehensive understanding of the mechanisms by which bacterial pathogens effectively infect their hosts.

关 键 词：ATTRACTIVE TRAPPING PROCESS 

分 类 号：R378[医药卫生—病原生物学]