基于网络药理学对七味白术散治疗菌群失调性腹泻的作用机制研究  

Study on the Mechanism of Qiwei Baizhu Powder in the Treatment of Dysbacterial Diarrhea Based on Network Pharmacology

作　　者：王威[1] 王静[1] WANG Wei;WANG Jing(The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine,National Research Center of Acupuncture and Moxibustion Clinical Medicine,Tianjin 300193)

机构地区：[1]天津中医药大学第一附属医院,国家中医针灸临床医学研究中心,天津300193

出　　处：《世界中西医结合杂志》2021年第7期1251-1255,1264,共6页World Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金面上项目(81873083)。

摘　　要：目的基于网络药理学的方法探讨七味白术散治疗菌群失调性腹泻(Flora imbalance diarrhea)的作用机制。方法通过中药系统药理学数据库(Traditional Chinese medicine system platform,TCMSP)获得七味白术散所含中药的主要化学成分,并收集其潜在作用靶点;菌群失调性腹泻疾病靶点来源于GeneCards、DrugBank、Home-OMIM-NCBI数据库。通过UniProt数据库对药物靶基因与疾病靶基因校正,使用Draw Venn Diagrams工具获得交集基因。应用Cytoscape 3.7.1对所得的交集基因进行PPI分析、GO与KEGG通路富集分析。结果共获得七味白术散活性成分84个,药物作用靶点201个,疾病靶点177个,交集基因26个。PPI网络中,丝裂原活化蛋白激酶1(mitogen-activated protein kinase 1,MAPK1)、Jun原癌基因(Jun proto-oncogene)AP-1转录因子亚基(AP-1 transcription factor subunit,JUN)、雌激素受体1(Estrogen receptor 1,ESR1)、糖原合成酶激酶3β(Glycogen synthase kinase 3,GSK3B)为关键靶点,GO富集分析和KEGG通路富集分析发现,药物有效成分主要通过白细胞介素-17信号通路、AGE-RAGE信号通路以及C型凝聚素受体等信号通路参与了细胞因子产生的正调控、DNA结合转录因子活性的调节、对脂多糖的反应等生物学过程。结论本研究揭示了七味白术散治疗菌群失调性腹泻可能是多成分、多靶点、多途径的,且为进一步研究提供思路和参考。Objective To discuss the mechanism of Action of Qiwei Baizhu powder in treatment of Flora imbalance diarrhea based on network pharmacology.Methods The main chemical constituents of Traditional Chinese medicine(TCM)in Qiwei Baizhu powder were obtained by systematic pharmacology database(TCMSP),and the potential targets were collected.Targets of bacterial colony dysregulatory diarrhea disease were derived from GeneCards,DrugBank and Home-Omim-NCBI databases.Intersection genes were obtained from Draw Venn Diagrams by modifying drug target genes and disease target genes based on UniProt database.The intersection genes were analyzed by PPI,GO and KEGG pathways by Cytoscape 3.7.1.Results A total of 84 active components,201 drug targets,177 disease targets and 26 intersection genes were obtained.In PPI network,mitogen-activated protein kinase 1(MAPK1),Jun proto-oncogene AP-1 transcription factor subunit(Jun),estrogen receptor 1(ESR1)and glycogen synthase kinase 3(GSK3B)were the key targets.GO enrichment analysis and KEGG pathway enrichment analysis showed that the active components of drugs were mainly involved in the positive regulation of cytokine production,the regulation of DNA-binding transcription factor activity,and the reaction to LPS and other biological processes through the interleukin-17 signaling pathway,AGE-RAGE signaling pathway and C-type coagulation receptor signaling pathway.Conclusion This study revealed that Qiwei Baizhu powder may be multi-component,multi-target and multi-pathway in the treatment of dysbacterial diarrhea,and provide ideas and references for further research.

关 键 词：七味白术散 菌群失调性腹泻 网络药理学 靶点 信号通路 

分 类 号：R285[医药卫生—中药学]