黑龙江省人源沙门菌流行特征及指纹图谱多态性分析  

Epidemiological characteristics and fingerprinting polymorphism of Salmonella in human,Heilongjiang

作　　者：闫军[1] 高飞[1] 李鑫[1] 薛成玉[1] 李敬晶 董锐[1] 郑晓华[1] 谢平会[1] 张剑峰[1] 遇晓杰 Yan Jun;Gao Fei;Li Xin;Xue Chengyu;Li Jingjing;Dong Rui;Zheng Xiaohua;Xie Pinghui;Zhang Jianfeng;Yu Xiaojie(Heilongjiang Provincial Center for Disease Control and Prevention,Harbin 150030,Heilongjiang,China;Hainan Provincial Center for Disease Control and Prevention,Haikou 570203,Hainan,China)

机构地区：[1]黑龙江省疾病预防控制中心,黑龙江哈尔滨150030 [2]海南省疾病预防控制中心,海南海口570203

出　　处：《疾病监测》2021年第6期605-610,共6页Disease Surveillance

基　　金：国家重点研发计划(No.2017YFC1601904)。

摘　　要：目的了解黑龙江省人源沙门菌的血清型及分子分型特征。方法对2016—2019年从黑龙江省监测腹泻病例分离的沙门菌进行血清学分型及脉冲场凝胶电泳(PFGE)图谱多态性分析。结果2016—2019年,9977例腹泻病例中共检出303株人源沙门菌,其中大庆、哈尔滨、齐齐哈尔市检出率较高,第二季度沙门菌检出率显著高于其他季度,16~及56~岁年龄组人群沙门菌检出率高于其他年龄组。黑龙江省人源沙门菌包含46种血清型,肠炎沙门菌占40.59%,为主要血清型。肠炎沙门菌共有67种PFGE谱型,型别多样,分为3个带型簇,54.10%的优势谱图菌株来源于≤5岁婴幼儿。结论16~及56~岁年龄组人群是人源沙门菌感染的高危人群,而肠炎沙门菌为主要血清型别。黑龙江省人源沙门菌指纹图谱呈多态性分布。Objective To understand the serotypes and molecular characteristics of Salmonella in human in Heilongjiang province.Methods Serotyping and pulsed-field gel electrophoresis(PFGE)polymorphism analysis on Salmonella isolated from diarrhea cases were carried out in Heilongjiang from 2016 to 2019.Results A total of 303 strains of human Salmonella were detected in 9977 cases of diarrhea patients during this period.The detection rates of Salmonella were higher in Daqing,Harbin and Qiqihar,and the annual detection rate was higher in the second quarter than in other quarters.The detection rates of Salmonella in age groups 16-20 years and 56-60 years were higher than those in other age groups.Salmonella in Heilongjiang had 46 serotypes.The proportion of S.enteritidis,the main serotype causing human infection,was as high as 40.59%.There were 67 different PFGE patterns of S.enteritidis,which can be divided into three band clusters.54.10%of the predominant strains were from infants under 5 years old.Conclusion People aged 16-20 years and 56-60 years were at high risk for human Salmonella infection,and S.enteritidis was the main serotype causing human infection.The fingerprints of human Salmonella in Heilongjiang showed polymorphism distribution.

关 键 词：沙门菌 流行特征 多态性分析 

分 类 号：R211[医药卫生—中医学] R387