宏基因组测序在腹泻暴发调查中的应用  

Application of metagenome sequencing in investigation of diarrhea outbreak

作　　者：崔志刚[1] 遇晓杰[2] 赵嘉咏[3] 张剑峰[2] 周海健[1] 李臻鹏 阚飙[1] 卢昕[1] Cui Zhigang;Yu Xiaojie;Zhao Jiayong;Zhang Jianfeng;Zhou Haijian;Li Zhenpeng;Kan Biao;Lu Xin(State Key Laboratory for Infectious Disease Prevention and Control,National Institute for Communicable Disease Prevention and Control,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Heilongjiang Provincial Center for Disease Control and Prevention,Harbin 150030,Heilongjiang,China;Henan Provincial Center for Disease Control and Prevention,Zhengzhou 450016,Henan,China)

机构地区：[1]中国疾病预防控制中心传染病预防控制所,传染病预防控制国家重点实验室,北京102206 [2]黑龙江省疾病预防控制中心,黑龙江哈尔滨150030 [3]河南省疾病预防控制中心,河南郑州450016

出　　处：《疾病监测》2021年第6期616-621,共6页Disease Surveillance

基　　金：国家科技重大专项(No.2018ZX10714002)。

摘　　要：目的评价宏基因组测序技术在腹泻暴发中的潜在应用价值。方法收集2017年报告的2例聚集性腹泻暴发事件的样品,针对样品进行多重荧光PCR检测;同时,应用宏基因组测序技术对样品进行测序,基于测序数据分析暴发事件的病原体。结果案例1采用多重荧光PCR检测,仅检测到副溶血弧菌;而采用宏基因组测序技术除检测到副溶血弧菌外,还检测到更高丰度的沙门菌。案例2采用多重荧光PCR未检测到病原体的样品,采用宏基因组测序技术检测到了志贺菌。结论宏基因组测序不需先验知识进行预判,可以避免引入误判,更有利于发现混合感染,而且在保证足够测序深度的情况下,可以获得比荧光PCR法更高的检测灵敏度。Objective To evaluate the potential application value of metagenome sequencing in the investigation of diarrhea outbreak.Methods The samples of two diarrhea outbreaks reported in 2017 were collected and detected by multiplex fluorescent PCR.Meanwhile,the isolates were sequenced by metagenome sequencing,and the potential pathogens of the outbreaks were analyzed based on the sequencing data.Results For outbreak 1,only Vibrio parahaemolyticus was detected by multiplex fluorescent PCR,while Salmonella with higher abundance was detected by metagenome sequencing in addition to V.parahaemolyticus.For outbreak 2,Shigella was detected by metagenome sequencing in samples with no pathogen detected by multiplex fluorescent PCR.Conclusion Metagenome sequencing needs no prediction of potential pathogens in samples and can avoid misdiagnosis,which is more conducive to the detection of mixed infection.Moreover,it has higher detection sensitivity compared with fluorescent PCR under the sufficient sequencing depth.

关 键 词：宏基因组测序 腹泻 暴发 

分 类 号：R211[医药卫生—中医学] R37