肠道病毒71型抗原检测板不同保存方法稳定性的比较  

Stabilities of enterovirus 71 antigen detection plate in dry and wet storages

作　　者：杨婷 李娅娴 李亚东 严皎 王永蓉 赵勇 文瑜 杜宋婷 刘正玲 YANG Ting;LI Ya-xian;LI Ya-dong;YAN Jiao;WANG Yong-rong;ZHAO Yong;WEN Yu;DU Song-ting;LIU Zheng-ling(Institute of Medical Biology,Chinese Academy of Medical Sciences,Kunming 650503,Yunnan Province,China)

机构地区：[1]中国医学科学院医学生物学研究所,云南昆明650503

出　　处：《中国生物制品学杂志》2021年第7期831-834,842,共5页Chinese Journal of Biologicals

基　　金：云南省重点新产品开发计划(2015BC008);中国医学科学院医学与健康科技创新工程(2016-12M-1-019)。

摘　　要：目的比较干燥保存及湿保存肠道病毒71型(enterovirus 71,EV71)抗原检测板的稳定性。方法用羊抗EV71多克隆抗体包被96孔酶标板,制备20块EV71抗原检测板,10块板置空气干燥后真空包装,-25℃保存;另外10块板用洗涤液浸泡,封口后置4℃保存。分别于保存0、20、40、60、90、120、180 d时,检测5份EV71样品。结果干燥保存EV71抗原检测板检测5份样品7个时间点的均值分别为315.0、299.4、7795.3、827.6、261.4 U/mL,湿保存的EV71抗原检测板检测结果均值分别为354.3、347.1、6766.6、749.7、277.3 U/mL,各时间点两种方法检测结果差异无统计学意义(P>0.05)。两种方法保存180 d时,EV71抗原检测板均可保持较好的活性(P>0.05)。结论两种方法保存EV71抗原检测板6个月内,均具有良好的稳定性。Objective To evaluate the stability of enteroviurs(EV)71 antigen detection plate in dry and wet storages.Methods Twenty EV71 antigen detection plates were coated with goat anti-EV71 polyclonal antibody,of which ten were dried in air then vacuum packed and stored at -25℃ while the other ten were soaked with washing liquid,then sealed and stored at 4℃.Five batches of EV71 samples were detected by the plates 0,20,40,60,90,120 and 180 d after storage.Results The mean detection results of samples 1~5 by the plates in dry storage at seven time points were 315.0,299.4,7795.3,827.6 and 261.4 U/mL,while those in wet storage were 354.3,347.1,6766.6,749.7 and 277.3 U/mL,respectively,which showed no significant difference(P>0.05).Both the detection plates showed high activities 180 d after storage(P>0.05).Conclusion Both EV71 antigen detection plates in dry and wet storages showed high stability within 6 months.

关 键 词：肠道病毒71型 抗原检测板 ELISA法 干燥保存 湿保存 稳定性 

分 类 号：R392-33[医药卫生—免疫学] R392.9[医药卫生—基础医学]