菌物代谢组学应用及存在的问题  

Application and Problems of Mtabolomics in Fungal Research

作　　者：尉杰 赵铖 陆瑞利[1] 胡丰林[1] WEI Jie;ZHAO Cheng;LU Ruili;HU Fenglin(Anhui Agricultural University,Engineering Research Center of Fungal Biotechnology Ministry of Education,Hefei 230036,China)

机构地区：[1]安徽农业大学真菌生物技术教育部工程研究中心,合肥230036

出　　处：《菌物研究》2021年第2期70-82,共13页Journal of Fungal Research

基　　金：国家自然科学基金项目(31570024);安徽省教育厅自然科学基金项目(KJ2015ZD21)。

摘　　要：代谢组学是一个可灵敏捕捉各种生物或非生物因素导致的供试样本中小分子代谢成分变化及其规律的实验方法,被广泛应用于生理生化、病理药理、生物工程、化学生态等很多方面。由于菌物种群数量庞大、未知结构次生代谢产物众多,严重影响到生物标记物的鉴定和进一步深入分析。为此,在总结目前菌物代谢组研究成果的基础上,结合十年的菌物代谢组学研究经验,提出如果要提高菌物代谢组学研究水平,就必须要加快菌物代谢物数据库以及基因组数据库的建设,同时,选择合理的代谢组学方法和多组学多方法联合分析也是现阶段提升菌物代谢组学研究水平的最可行的办法之一。Metabolomics is an experimental method which can sensitively capture the small molecular changes and regularity in testing samples caused by various biotic or abiotic factors.It is widely used in physiology,biochemistry,pathology,pharmacology,bioengineering,chemical ecology and many other fields.Fungi have a large population size and contain large number of unknown secondary metabolites which severely limited identification and further analysis of the biomarkers.Therefore,based on summarizing metabolomics literatures of fungi and ten years’experience in fungal metabolomics research,the author suggest that it is necessary to speed up the construction of the database of metabolites and genome database of fungi to improve the level of metabolomics research.Meanwhile,rational selection of metabolomics methods and using combined omics is also one of the most feasible ways to improve the level of metabolomics research at present.

关 键 词：代谢组学 菌物 代谢组学研究方法 酵母代谢组学 病原菌代谢组学 

分 类 号：Q949[生物学—植物学] Q493