转玉米PEPC基因水稻的创建及其农艺性状分析  

Generation of pCab::PEPC transgenic rice and analysis of its yield, quality and stress tolerance

作　　者：王振 魏珂 周豫 辛世文[2] 刘巧泉[1] 李钱峰[1] WANG Zhen;WEI Ke;ZHOU Yu;XIN Shiwen;LIU Qiaoquan;LI Qianfeng(Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/College of Agriculture,Yangzhou University,Yangzhou 225009,China;The Chinese University of Hong Kong,Hong Kong 999077,China)

机构地区：[1]扬州大学农学院/江苏省作物基因组学和分子育种重点实验室/植物功能基因组学教育部重点实验室/江苏省粮食作物现代产业技术协同创新中心,江苏扬州225009 [2]香港中文大学,香港999077

出　　处：《扬州大学学报（农业与生命科学版）》2021年第3期90-96,共7页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家自然科学基金资助项目(32071984);江苏省自然科学基金杰出青年基金项目(BK20200045);江苏省“六大人才”高峰项目(SWYY-154)。

摘　　要：将水稻Cab基因启动子与来自玉米的PEPC基因融合后通过农杆菌介导的转化法转入粳稻品种武香粳9号中,经分子鉴定和表达分析明确PEPC基因成功转入水稻并获表达。对pCab::PEPC转基因水稻的产量和品质性状进行了系统分析,发现其具有较好的增产潜力,且对稻米品质有一定的改良作用。此外,pCab::PEPC转基因水稻在苗期和种子萌发阶段均表现出良好的非生物胁迫抗性。综上所述,通过选择合适的启动子驱动PEPC基因在水稻中特异性表达,可显著增强水稻产量、品质和抗逆性,具有良好的育种应用前景。The promoter of Cab gene of rice was fused with the maize PEPC gene, and then transferred into japonica rice Wuxianggeng 9 via Agrobacterium-mediated transformation. The results of genotyping and expression analysis indicated that PEPC was successfully transformed and expressed in rice. The analysis of rice yield and quality related traits suggested that overexpression of PEPC in rice not only increased rice yield but also promoted rice quality. Moreover, pCab::PEPC transgenic rice performed much better than the wild-type control in response to abiotic stress in the stages of both seedling growth and seed germination. In brief, selection of appropriate promoter to drive the expression of PEPC gene in rice could promote rice yield and quality, as well as its stress tolerance, thus has potential application in future rice breeding programs.

关 键 词：水稻 PEPC基因 Cab启动子 产量 稻米品质 非生物胁迫 种子萌发 

分 类 号：S511[农业科学—作物学] Q786[生物学—分子生物学]