ABP1参与BY2细胞生长素响应的Ca^(2+)信号转导过程  

ABP1 is involved in auxin signaling and Ca^(2+) response transduction in BY2

作　　者：徐茂盛 赵燕[1] 翟翔 廖李玲 黄妤[1] 黄丽华[1] 陈金军[1] 张学文[1] XU Maosheng;ZHAO Yan;ZHAI Xiang;LIAO Liling;HUANG Yu;HUANG Lihua;CHEN Jinjun;ZHANG Xuewen(College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha 410128,China)

机构地区：[1]湖南农业大学生物科学技术学院,长沙410128

出　　处：《扬州大学学报（农业与生命科学版）》2021年第3期117-121,128,共6页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：湖南省教育厅重点研究项目(18A104)。

摘　　要：将Ca^(2+)响应实时荧光报告系统引入雌二醇诱导生长素结合蛋白(ABP1)调控表达的BY2细胞中,获得了ABP1过表达(ABP1-ox)、抑制表达(ABP1-anti)同时对Ca^(2+)标记的几个BY2细胞株。对这些细胞株进行雌二醇诱导调控其ABP1过表达或抑制表达后,分析ABP1的表达量,并通过在细胞外添加IAA处理,实时观察ABP1调控表达后细胞的Ca^(2+)信号变化。结果表明:ABP1-ox细胞在雌二醇诱导后细胞内ABP1表达量显著提高,细胞经IAA处理后,细胞膜内、核膜周围均有迅速而强烈的荧光信号,说明ABP1过表达后细胞能快速响应胞外的IAA作用,将信号转导到细胞内,使细胞质内Ca^(2+)信号迅速增强;而ABP1-anti细胞经雌二醇诱导后,细胞内ABP1表达显著下调,细胞经IAA处理后,相对于对照,细胞内的荧光信号微弱且呈点状,细胞核附近的荧光不明显,说明细胞内Ca^(2+)信号的转导受到抑制。这证明BY2细胞中ABP1参与生长素信号与细胞内Ca^(2+)响应的信号转导过程。A real-time Ca^(2+)signal observation system was introduced into BY2 cells in which the ABP1(auxin binding protein 1) is regulated by estradiol inducible expression. Thus the several cell lines were obtained of ABP1 overexpression(ABP1-ox), inhibited expression(ABP1-anti) as well as the Ca^(2+) signal was labeled by fluorescence. The Ca^(2+) signal was observed combined with the IAA treatment afterABP1 expression regulation in those cell lines. The ABP1 expression was apparently increased by estradiol introducing in ABP1-ox cells and the real-time observation showed that the fluorescence signals accumulated around cell membrane and surrounding the nucleus that was quickly response to the indole 3-acetic acid(IAA) treatment, which demonstrated that the cell could rapidly respond to the extracellular IAA and transduce the signals into Ca^(2+) response in ABP1-ox cell. The ABP1 expression was in a reduced level in the ABP1-anticell after inducing. Only weak fluorescence signal in point was observed in cell seems unable to effectively transfer the signal to the cell nucleus when treated with IAA in ABP1-anti cell. The results confirm that ABP1 is involved in auxin response Ca^(2+)signaling in BY2 cell.

关 键 词：ABP1 生长素信号途径 Ca^(2+)信号转导 BY2细胞 

分 类 号：Q257[生物学—细胞生物学]