一种改进型麦克马斯特虫卵计数板的应用效果研究  

Application Evaluation of a Modified McMaster Egg Counting Plate

作　　者：韩利方[1] 邹均平 谢月华 刘易扬 闫文朝[1] Han Lifang;Zou Junping;Xie Yuehua;Liu Yiyang;Yan Wenchao(College of Animal Science and Technology,Henan University of Science and Technology,Luoyang Henan 471023;Veterinary Inspection Institute,Agriculture and Rural Affairs Bureau of Babu District,Hezhou Guangxi 542899)

机构地区：[1]河南科技大学动物科技学院,河南洛阳471023 [2]广西贺州市八步区农业农村局动物卫生监督所,广西贺州542899

出　　处：《中国农学通报》2021年第21期145-150,共6页Chinese Agricultural Science Bulletin

基　　金：河南省科技攻关计划项目“肉孢子虫高效检测技术的研发”(172102110017);河南省自然科学基金项目“中国猪源肉孢子虫种类分布特征及其遗传多态性研究”(182300410088)。

摘　　要：为了评价一种改进型麦克马斯特虫卵计数板的性能,利用改进型麦克马斯特虫卵计数板和经典麦克马斯特虫卵计数板对兔粪便样品和球虫卵囊悬液样品进行计数和比较分析。结果显示,用饱和氯化钠溶液作为漂浮液,改进型麦克马斯特虫卵计数板能对卵囊悬液样品和2~5 g固体粪便样品中球虫卵囊进行准确计数,与麦克马斯特虫卵计数板计数结果的符合率为95.59%~99.49%;用自来水作为悬浮液,改进型麦克马斯特虫卵计数板能对卵囊悬液样品和0.3~0.5 g固体粪便样品中球虫卵囊进行准确计数。本研究发现,改进型麦克马斯特虫卵计数板对临床和实验室样品中虫卵能进行准确计数,而且比经典麦克马斯特虫卵计数板具有更强大的检测功能。To evaluate the performance of modified McMaster egg counting plate developed in our lab,fecal samples of rabbits and oocyst suspension of Eimeria tenella were examined and counted using both classic and modified McMaster egg counting plates.The results of floating methods with saturated sodium chloride solution showed that the numbers of oocysts in rabbit fecal samples(2-5 g)and oocyst suspension were precisely counted using the modified McMaster plate.The coincidence rate with the results of classic McMaster plate was 95.59%-99.49%.In the precipitation experiment with tap water,the oocyst numbers in rabbit fecal samples(0.3-0.5 g)and oocyst suspension were also precisely counted using the modified McMaster plate.These findings suggest that the modified McMaster egg counting plate could be used to count the oocyst or egg number in fecal specimen and suspensions in labs,and is more powerful in counting functions than the classic McMaster plate.

关 键 词：改进型麦克马斯特虫卵计数板 粪样 虫卵悬液 虫卵 卵囊 包囊 定量分析 

分 类 号：S855.9[农业科学—临床兽医学]