药用植物滇黄精寄生真菌的分离与鉴定  

Isolation and Identification of Parasitic Fungi from Polygonatum kingianum

作　　者：谭伟 浦仕彪 Tan Wei;Pu Shibiao(Yunnan University of Chinese Medicine,Kunming 650500)

机构地区：[1]云南中医药大学,昆明650500

出　　处：《中国农学通报》2021年第19期90-94,共5页Chinese Agricultural Science Bulletin

基　　金：云南省高层次人才培养支持计划“云南省万人计划青年拔尖人才专项-浦仕彪”(YNWR-QNBJ-2018-190);云南省科学技术厅人才与平台计划省级工程技术研究中心建设项目“云南省道地濒危中药材繁育与栽培工程技术研究中心”(2016DH011)。

摘　　要：为了解滇黄精寄生真菌及其优势居群,采集滇黄精种植区云南省丽江地区玉龙县和华坪县的样品,采用组织分离法、样品保湿法和三点接种法分别对滇黄精寄生真菌进行分离、培养和纯化。对纯化菌株进行传统形态学及分子生物学鉴定。玉龙县鲁甸乡采集到15株寄生真菌(1株青霉属Penicillium、12株尖孢镰刀菌Fusarium oxysporum、1株木贼镰刀菌Fusarium equiseti、1株链格孢菌Alternaria alternata),华坪县采集到22株滇黄精寄生真菌(12株黑绿木霉Trichoderma atroviride、9株芬芳镰刀菌Fusarium redolens、1株木贼镰刀菌Fusarium equiseti)。2个试验地所采集的滇黄精镰刀属真菌菌株占比较大,为优势寄生真菌,研究结果将为滇黄精病害防治提供理论依据。The purpose of this study is to understand the parasitic fungi and their dominant populations.Samples were collected from Yulong County and Huaping County in Lijiang of Yunnan Province. The fungi were isolated, cultured and purified by tissue separation, sample moisture preservation and three-point inoculation method, respectively. The purified strains were identified by combining traditional morphological and molecular biological identification. Fifteen strains of parasitic fungi (1 strain of Penicillium, 12 strains of Fusarium oxysporum, 1 strain of Fusarium equiseti, and 1 strain of Alternaria alternata) were identified in Ludian township of Yulong County. A total of 22 fungal isolates (12 strains of Trichoderma atroviride, 9 strains of Fusarium redolens, and 1 strain of Fusarium equiseti) were found in Huaping County. The strains of Fusarium are the dominant parasitic fungi in the samples collected from the two test places. The results could provide a theoretical basis for the disease control of Polygonatum kingianum.

关 键 词：滇黄精 真菌 分离 鉴定 内转录间隔区 

分 类 号：S859.84[农业科学—临床兽医学]