新型冠状病毒核酸检测结果的影响因素  

Factors influencing the results of 2019-nCoV nucleic acid test

作　　者：霍雨佳 潘丽峰[1] 崔琪奇 刘青[1] 袁洋[1] 郝莉鹏 Huo Yujia;Pan Lifeng;Cui Qiqi;Liu Qing;Yuan Yang;Hao Lipeng(Pudong New District Center for Disease Control and Prevention,Shanghai 200136,China)

机构地区：[1]上海市浦东新区疾病预防控制中心,200136

出　　处：《中华实验和临床病毒学杂志》2021年第3期345-348,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：国家科技重大专项(2018ZX10713001-008);上海市浦东新区疾病预防控制中心卫生科技项目(PDCDC-2020-02);上海市浦东新区疾病预防控制中心后备学科带头人项目(PDCDC-HBXD2020-04)。

摘　　要：新型冠状病毒肺炎(Coronavirus Disease 2019,COVID-19)是由新型冠状病毒(2019 novel Coronavirus,2019-nCoV)引起的传染性疾病。目前确诊2019-nCoV使用最多的方法是核酸检测法,疑似病例具备实时荧光RT-PCR检测2019-nCoV核酸阳性者即为确诊病例。在核酸检测过程中,标本质量、标本运输和保存条件、检测试剂和方法等因素都有可能造成假阴性或者假阳性结果。本文综述2019-nCoV核酸检测过程中可能影响检测结果的因素,更好地为COVID-19疑似病例确诊提供依据。Coronavirus Disease 2019(COVID-19)is an infectious disease caused by 2019 novel Coronavirus(2019-nCoV).At present,the most used method to diagnose 2019-nCoV is nucleic acid test.Suspected cases underwent real-time fluorescent RT-PCR examination,and those who were 2019-nCoV nucleic acid positive were confirmed cases.However,in the process of nucleic acid test,factors such as specimen quality,specimen transportation and storage conditions,detection reagents and methods may cause false negative or false positive results.This article reviews the factors that may affect the results of 2019-nCoV nucleic acid test,and provides a basis for the diagnosis of suspected COVID-19 cases.

关 键 词：新型冠状病毒肺炎 新型冠状病毒 核酸检测 

分 类 号：R563.1[医药卫生—呼吸系统] R440[医药卫生—内科学]