基于香豆素-NBD体系的荧光探针用于选择性检测和区分半胱氨酸、高半胱氨酸和谷胱甘肽  

Fluorescent probe based on coumarin-NBD system is used to selectively detect and distinguish Cys,Hcy and GSH

作　　者：仝红娟 赵梅梅 杜漠 TONG Hong-juan;ZHAO Mei-mei;DU Mo(School of Pharmacy,Shaanxi Institute of International Trade&Commerce,Xi'an 712046,China;Collaborative Innovation Center of Green Manufacturing Technology for Traditional Chinese Medicine in Shaanxi Province,Xi'an 712046,China)

机构地区：[1]陕西国际商贸学院医药学院,陕西西安712046 [2]陕西省中药绿色制造技术协同创新中心,陕西西安712046

出　　处：《精细与专用化学品》2021年第7期13-17,共5页Fine and Specialty Chemicals

基　　金：陕西省教育厅2020年度专项科学研究计划(20JK0510);陕西省自然科学基础研究计划(2019JQ-924);陕西省中药绿色制造技术协同创新中心重点培育项目(2019XT-1-03)。

摘　　要：设计合成了一个可检测和区别半胱氨酸(Cys)、高半胱氨酸(Hcy)以及谷胱甘肽(GSH)的荧光探针1。通过将染料7-硝基苯并-2-氧杂-1,3-二唑(NBD)和马来酰亚胺基团引入3-氨基-7-羟基香豆素的两端,利用双重PET和ICT机理来降低探针自身荧光背景,同时结合2个不同的荧光团来提高选择性。此探针不仅易于制备,而且可以在温和的条件下,在水溶液中高选择性和高灵敏性的检测和区分Cys、Hcy以及GSH。Fluorescent probe 1 was designed and synthesized,which can simultaneously detect and distinguish among Cys,Hcy and GSH,by adding both 4-nitro-2,1,3-benzoxadiazol(NBD)and maleimide groups onto the ends of the fluorophore 3-amino-7-hydroxycoumarin.The probe features dual-PET and ICT mechanisms was considered to reduce the fluorescence background and dual cyclizations to improve selectivity.The probe was conveniently prepared and could detection and differentiate these important aminothiols with high selectivity and high sensitivity in the aqueous solution under mild conditions.

关 键 词：荧光探针 检测 氨基硫醇 

分 类 号：TQ422[化学工程]