酶促水凝胶的合成及生物传感应用研究进展  

Synthesis of enzymatic hydrogels and their application in biosensors

作　　者：苗向阳[1,3] 潘佳 朱倩 朱皓雨 韦天香 Xiangyang Miao;Jia Pan;Qian Zhu;Haoyu Zhu;Tianxiang Wei(Department of Medical Science and Technology.Suzhou Chien-shiung Institute of Technology,Taicang 215411,China;School of Environment,Nanjing Normal University,Nanjing 210023,China;School of Chemistry and Materials Science,Nanjing Normal University,Nanjing 210023,China)

机构地区：[1]苏州健雄职业技术学院生物医药学院,太仓215411 [2]南京师范大学环境学院,南京210023 [3]南京师范大学化学与材料科学学院,南京210023

出　　处：《中国科学：化学》2021年第7期919-931,共13页SCIENTIA SINICA Chimica

基　　金：国家自然科学基金(编号:21705079,22074064);江苏省自然科学基金(编号:BK20171033)资助项目。

摘　　要：近年来,聚合物基水凝胶材料已经成为一种独特的修饰材料,为新型生物传感器的设计提供了新思路.酶具有特异性高、毒性低、催化效率高等良好特性,已被用于催化聚合各种新型水凝胶.本文综述了几种酶促水凝胶的合成方法及其在细胞代谢物、组织工程、伤口愈合和癌症监测等方面的潜在应用,并对基于酶促水凝胶反应的生物传感器的制备与应用进行了总结与展望.In recent years,polymer-based hydrogels have become the unique electrode modification material,which provides a new idea for the design of new biosensors.Furthermore,enzymes have been used to catalyze the polymerization of various hydrogels due to their high specificity,low toxicity and high catalytic efficiency.In this paper,we discussed several synthesis methods of enzymatic hydrogels and their potential application in cell metabolites,tissue engineering,wound healing,and cancer surveillance.Concluding remarks and future prospects regarding preparation and application of biosensor based on enzymatic gel reaction were provided at the end.

关 键 词：水凝胶 酶促聚合 生物传感 酶固定化 生物分子检测 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置] TQ427.26[自动化与计算机技术—控制科学与工程]