Molecular taxonomy of the primate amygdala via single-nucleus RNA sequencing analysis  

单细胞核RNA测序描绘猕猴杏仁核神经细胞类群图谱

作　　者：Lei Zhang Yanyong Cheng Shihao Wu Yufeng Lu Zhenyu Xue Xiao Chen Dai Chen Bo Zhang Zilong Qiu Hong Jiang 张磊;程燕咏;吴诗昊;卢玉峰;薛震宇;陈潇;陈岱;张博;仇子龙;姜虹(Department of Anesthesiology,Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200011,China;Institute of Neuroscience,State Key Laboratory of Neuroscience,CAS Key Laboratory of Primate Neurobiology,CAS Center for Excellence in Brain Science and Intelligence Technology,University of Chinese Academy of Sciences,Chinese Academy of Sciences,Shanghai 200031,China;State Key Laboratory of Brain and Cognitive Science,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China;University of Chinese Academy of Sciences,Beijing 100049,China;Shanghai Novel Bio-Pharm Technology Co.,LTD,Shanghai 201114,China;Kunming Institute of Zoology,Chinese Academy of Sciences,Kunming 650223,China;National Clinical Research Center for Aging and Medicine,Huashan Hospital,Fudan University,Shanghai 200040,China)

机构地区：[1]Department of Anesthesiology,Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200011,China [2]Institute of Neuroscience,State Key Laboratory of Neuroscience,CAS Key Laboratory of Primate Neurobiology,CAS Center for Excellence in Brain Science and Intelligence Technology,University of Chinese Academy of Sciences,Chinese Academy of Sciences,Shanghai 200031,China [3]State Key Laboratory of Brain and Cognitive Science,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China [4]University of Chinese Academy of Sciences,Beijing 100049,China [5]Shanghai Novel Bio-Pharm Technology Co.,LTD,Shanghai 201114,China [6]Kunming Institute of Zoology,Chinese Academy of Sciences,Kunming 650223,China [7]National Clinical Research Center for Aging and Medicine,Huashan Hospital,Fudan University,Shanghai 200040,China

出　　处：《Science Bulletin》2021年第14期1379-1383,M0003,共6页科学通报（英文版）

基　　金：the National Natural Science Foundation of China(82071177,81970990,81771132,31625013,and 91732302);Shanghai Brain-Intelligence Project from STCSM(16JC1420501);the Strategic Priority Research Program of the Chinese Academy of Sciences(XDBS01060200);the Shanghai Municipal Science and Technology Major Project(2018SHZDZX05);the Open Large Infrastructure Research of Chinese Academy of Sciences,the National Key R&D Program of China(2017YFA0105201);the CAMS Innovation Fund for Medical Sciences(CIFMS 2016-I2M-2-001);the Shanghai Jiao Tong University School of Medicine Two-hundred Talent(20191818);the Cross Disciplinary Research Fund of Shanghai Ninth People’s Hospital(JYJC202002);the Key Scientific and Technological Projects of Guangdong Province(2018B030335001)。

摘　　要：The amygdala is an evolutionarily conserved brain region found in vertebrates.Accumulated pieces of evidence over the past few decades strongly suggest that the amygdala is involved in a wide range of behavioral responses,including social,aggressive,and parenting behaviors[1,2].In particular,amygdala dysfunction in humans is associated with psychiatric and emotional disorders[3,4].Although intensive studies have been carried out to analyze the role of the amygdala in rodents,the anatomical structure of the amygdala has changed enormously between rodents and primates during evolution[1].Unlike in rodent brains,the basal and lateral amygdala(BLA)encompasses the major regions in the primate brain,compared to the central amygdala(CeA)and medial amygdala(MeA)[1,5].The developmental and regional gene profiling of the amygdala in rhesus monkeys was previously performed[6].However,the gene expression information acquired through single-cell resolution is still not available.在哺乳动物中,杏仁核是控制情绪反应和管理疼痛应激等生理功能的核心脑功能区域.本研究使用高通量单细胞核RNA的测序方法,首次描绘了幼年恒河猕猴的杏仁核神经细胞类群图谱.恒河猕猴杏仁核兴奋性神经元中,只有SLC17A7(VGLUT1)是主要表达的标志物,SLC17A6(VGLUT2)表达较弱;而在抑制性神经元中,两种标志物GAD1和GAD2均普遍表达.五种主要的抑制性神经元各自占比不同:CCK:30%,SST:20%,NOS1:35%,TAC1:10%,VIP:5%.确定了灵长类动物特有的两种新的星形胶质细胞亚型(AS4和AS5),NPSR1选择性地富集在AS4细胞群,而T8SIA2和SEMA3C则选择性地在AS5细胞群中高表达.物种间的基因表达差异分析发现,与小鼠不同的是,NHS选择性地在猕猴NOS1阳性的抑制性神经元中高表达,SERPINI1选择性地在猕猴少突胶质细胞中高表达.

关 键 词：情绪反应 少突胶质细胞 灵长类动物 杏仁核 星形胶质细胞 细胞群 神经细胞 基因表达差异 

分 类 号：Q421[生物学—神经生物学]