黑羽乌蒙乌骨鸡ESR2、PRL基因多态性与产蛋数的关联性分析  

Correlation analysis of ESR2 and PRL genes polymorphisms with egg production of black-feather Wumeng silky fowls

作　　者：陈泽林 曾姗姗 欧茂均 张勇[2] 王天松 郭徵力 叶红英 张习本 李未博 张建英 张林达 李霞 罗梦阳 向振博 CHEN Zelin;ZENG Shanshan;OU Maojun;ZHANG Yong;WANG Tiansong;GUO Zhengli;YE Hongying;ZHANG Xiben;LI Weibo;ZHANG Jianying;ZHANG Linda;LI Xia;LUO Mengyang;XIANG Zhenbo(Bijie Institute of Animal Husbandry and Veterinary Medicine,Bijie 551700,China;College of Animal Science,Guizhou University,Guiyang 550025,China)

机构地区：[1]毕节市畜牧兽医科学研究所,贵州毕节551700 [2]贵州大学动物科学学院,贵阳55002

出　　处：《黑龙江畜牧兽医》2021年第14期128-136,158,159,共11页Heilongjiang Animal Science And veterinary Medicine

基　　金：毕节市科技计划项目“黑羽乌蒙乌骨鸡选育与推广利用”(毕科合字〔2016〕16号)。

摘　　要：为了探究黑羽乌蒙乌骨鸡ESR2和PRL基因多态性及其对产蛋数的影响,试验采用PCR直接测序技术筛选黑羽乌蒙乌骨鸡ESR2和PRL基因单核苷酸多态性(SNP)位点,对ESR2、PRL基因mRNA二级结构和蛋白质二级、三级结构进行预测,并分析SNP位点的遗传特性、连锁不平衡及单倍型和双倍型,最后对ESR2、PRL基因SNP位点不同基因型和双倍型、合并基因型与300日龄黑羽乌蒙乌骨鸡产蛋数的关联性进行分析。结果表明:在黑羽乌蒙乌骨鸡ESR2基因中检测到3个SNP位点,均产生3种基因型,分别为位于内含子7的g.53223142 G>A,内含子8的g.53223404 G>A,外显子8的g.53223338 C>T;其中外显子8的g.5322338 G>T,导致苏氨酸变为甲硫氨酸,为错义突变位点。在PRL基因中检测到4个SNP位点,分别为位于内含子4的g.58576867 A>G,位于外显子5的g.58577099 C>T、g.58577160 C>G和g.58577368 T>C,其中位于外显子5的g.58577099 C>T位于外显子编码区,而其他两个位点位于外显子非编码区。ESR2基因SNP位点g.53223338 C>T,突变前mRNA序列二级结构的自由能为-1 789.92 kJ/mol,突变后自由能为-1 794.10 kJ/mol;蛋白质二级结构中的α-螺旋、β-转角、无规卷曲和延伸链占比和三级结构都不发生改变。ESR2基因3个SNP位点中的优势基因型分别为AA、TT、GA,相对应的优势等位基因分别为A、T和A,3个SNP位点均未偏离Hardy-Weinberg平衡(P>0.05);PRL基因4个SNP位点中的优势基因型分别为AA、TT、GG、TC,相对应的优势等位基因分别为A、T、G、T,SNP位点g.58576867 A>G、g.58577099 C>T和g.58577160 C>G未偏离Hardy-Weinberg平衡(P>0.05),g.58577368 T>C显著偏离Hardy-Weinberg平衡(P<0.05)。ESR2基因3个SNP位点间均不存在强连锁不平衡,3个SNP位点中存在4种单倍型、8种双倍型;PRL基因4个SNP位点中g.58577099 C>T与g.58577160 C>G之间完全连锁不平衡,其他位点间均不存在强连锁不平衡,4个SNPs位点中存在4种单倍型、8种双倍型。300日�In order to explore the ESR2 and PRL genes polymorphisms of black-feather Wumeng silky fowls and their effect on egg production,the experiment used PCR direct sequencing technology to identify the single nucleotide polymorphisms(SNP)locus of ESR2 and PRL genes of black-feather Wumeng silky fowls.The mRNA secondary structure and protein secondary and tertiary structure of ESR2 and PRL genes were predicted,and the genetic characteristics,linkage disequilibrium,haplotypes and diplotypes of SNPs were analyzed.The correlation between different genotypes,diplotypes and combined genotypes of ESR2 and PRL genes SNP locus and egg production of 300-day-old black feather Wumeng silky fowls were analyzed.The results showed that there were three SNPs in the ESR2 gene of the black-feather Wumeng silky fowls,all of which produced three genotypes and were located at g.53223142 G>A in intron 7,g.53223404 G>A in intron 8,g.53223338 C>T in exon 8.Among them,g.53223338 C>T in exon 8 causing threonine to change to methionine,which was a missense mutation.There were 4 SNPs in the PRL gene,located at g.58576867 A>G in intron 4,g.58577099 C>T,g.58577160 C>G and g.58577368 T>C in exon 5.Among them,g.58577099 C>T in exon 5 located in the exon coding region.,and the other two sites located in the exon noncoding region.The free energy of mRNA sequence secondary structure of the ESR2 gene g.53223338 C>T was-1789.92 kJ/mol before mutation,and-1794.10 kJ/mol after mutation.Theαhelix,βturn,random coil,extended chain in secondary structure and tertiary structure of the ESR2 protein were not changed.The dominant genotypes of the three SNPs in ESR2 gene were AA,TT and GA,and the corresponding dominant alleles were A,T and A,respectively.The three SNPs did not deviate from Hardy-Weinberg equilibrium(P>0.05).The dominant genotypes of the four SNPs in PRL gene were AA,TT,GG,TC,and the corresponding dominant alleles were A,T,G,T,respectively.SNPs g.58576867 A>G,g.58577099 C>T and g.58577160 C>G did not deviate from Hardy-Weinberg equilibrium(P>0.05)

关 键 词：繁殖性能 ESR2基因 PRL基因 单核苷酸多态性(SNP) 产蛋数 关联性分析 

分 类 号：S831.2[农业科学—畜牧学]