应用流式细胞术检测胃癌移植瘤小鼠脾脏中MDSC及T淋巴细胞亚群的变化  

Application of Flow Cytometry to Detect the Changes of MDSC and T Lymphocyte Subsets in the Spleen of Mice with Gastric Cancer Transplantation

作　　者：李南 曾凡业[1] 仝梦婷 节阳华[1] 轩艳红 张洪亮[1] LI Nan;ZENG Fan-ye;TONG Meng-ting;JIE Yang-hua;XUAN Yan-hong;ZHANG Hong-liang(Second Department of Oncology,the Fourth Affiliated Hospital of Xinjiang Medical University,Urumqi 830000,Xinjiang,China)

机构地区：[1]新疆医科大学第四附属医院肿瘤二科,新疆乌鲁木齐830000

出　　处：《医学信息》2021年第16期77-80,共4页Journal of Medical Information

基　　金：新疆维吾尔自治区自然科学基金项目(编号:2016D01C134)。

摘　　要：目的观察胃荷瘤小鼠脾脏中髓源性抑制细胞(MDSC)、调节性T细胞(Treg)和传统T淋巴细胞群的变化。方法随机将30只C57BL/6J小鼠分成荷瘤组和正常组,荷瘤组小鼠模型右侧背部皮下注射小鼠前胃癌细胞,正常组注射等量生理盐水,待肿瘤形成后,采用多色流式分析检测两组小鼠脾脏中MDSC、Treg以及CD4^(+)和CD8^(+)T细胞数量和相应比例,膜联蛋白-V(Annexin-V)染色检测CD4^(+)和CD8^(+)T细胞凋亡变化,5-溴脱氧尿嘧啶核苷(BrdU)染色检测CD4^(+)和CD8^(+)T细胞增殖变化。结果荷瘤组小鼠脾脏中MDSC和Treg占总脾脏细胞中的数量和比例高于正常组,CD4^(+)和CD8^(+)T细胞数量和比例低于正常组,差异有统计学意义(P<0.05)。荷瘤组小鼠脾脏中CD4^(+)和CD8^(+)T细胞增殖低于正常组,CD8^(+)T细胞凋亡高于正常组,差异有统计学意义(P<0.05)。结论MDSC和Treg细胞可积聚在胃荷瘤小鼠脾脏中,且其能抑制CD4^(+)和CD8^(+)T细胞增殖和促进CD8^(+)T细胞凋亡。Objective To observe the changes of myeloid-derived suppressor cells(MDSC),regulatory T cells(Treg)and traditional T lymphocyte populations in the spleen of mice bearing gastric tumors.Methods A total of 30 C57BL/6J mice were randomly divided into tumor-bearing group and normal group.The right back of the tumor-bearing group was injected with mouse forestomach carcinona cell,and the normal group was injected with the same amount of normal saline.After the tumor formed,polychromatic flow was used.Formula analysis was used to detect the number and corresponding proportions of MDSC,Treg,CD4^(+)and CD8^(+)T cells in the spleens of the two groups of mice.Annexin-V(Annexin-V)staining was used to detect the changes of CD4^(+)and CD8^(+)T cell apoptosis,5-bromodeoxy Uracil(BrdU)staining was used to detect the proliferation of CD4^(+)and CD8^(+)T cells.Results The number and proportion of MDSC and Treg in the total spleen cells in the spleen of the tumor-bearing mice were higher than that of the normal group,and the number and proportion of CD4^(+)and CD8^(+)T cells were lower than the normal group,the difference was statistically significant(P<0.05).The proliferation of CD4^(+)and CD8^(+)T cells in the spleen of mice in the tumor-bearing group was lower than that in the normal group,and the apoptosis of CD8^(+)T cells was higher than that in the normal group,the difference was statistically significant(P<0.05).Conclusion MDSC and Treg cells can accumulate in the spleen of gastric tumor-bearing mice,and they can inhibit the proliferation of CD4^(+)and CD8^(+)T cells and promote the apoptosis of CD8^(+)T cells.

关 键 词：骨髓源性抑制细胞 T淋巴细胞亚群 胃癌 CD4+T细胞 CD8+T细胞 

分 类 号：R392[医药卫生—免疫学]