菠菜色素的提取与分离实验教学优化探索  

Exploration on the Optimization of Spinach Pigment Extraction and Separation Experiment Teaching

作　　者：郭乃妮[1] 郑敏燕[1] 赵维[1] 纪思媛 曾睿 李敏 UO Naini;ZHENG Minyan;ZHAO Wei;JI Siyuan;ZENG Rui;LI Min(College of Chemistry and Chemical Engineering,Xianyang Normal University,Xianyang 712000,China)

机构地区：[1]咸阳师范学院化学与化工学院,陕西咸阳712000

出　　处：《皮革与化工》2021年第4期41-44,共4页Leather And Chemicals

基　　金：咸阳市科学技术研究计划项目(2019k02-22);咸阳师范学院教育教学改革研究项目(2019Y024);咸阳师范学院大学生创新创业基地项目(XSYC202117)。

摘　　要：天然产物的提取和分离是有机实验课程中重要的实验项目之一。萃取、蒸馏、柱层析等化学方法与波谱技术的结合使然产物的提取和分离研究大为方便。但是在柱层析实验操作中,存在着液体色素添加不均匀、时间受限、层析效果不佳等问题,特别是对菠菜色素的提取与分离实验中叶黄素、叶绿素和胡萝卜素的分离影响最大。研究通过采用菠菜色素的提取浓缩液和中性氧化铝的混合法,可以有效地提高柱层析色素的分离效果,使实验快速、精确完成,有助于培养学生的实验创新能力。The extraction and separation of natural products is one of the important experimental projects in the organic experiment course.The combination of chemical methods such as extraction,distillation,column chromatography and spectral technology is very convenient for the extraction and separation of the products.However,in the column chromatography experiment,there are problems such as uneven addition of liquid pigment,limited time and poor chromatographic effect,in particular,the separation of lutein,chlorophyll and carotene in the extraction and separation experiment of spinach pigment has the greatest influence.The optimal combination of concentrated extract solution of spinach pigment and neutral alumina was studied,which can effectively improve the column chromatography pigment separation effect,so that the experiment was completed quickly and accurately,the students'experimental innovation ability is cultivated.

关 键 词：菠菜色素 提取 分离 柱层析 

分 类 号：O627[理学—有机化学]