大豆孢囊线虫生防菌株Myrothecium verrucaria ZW-2发酵条件优化及活性物质分析  

Optimization of Fermentation Conditions of Myrothecium verrucaria ZW-2,a Biocontrol Strain Against Heterodera glycines and Analysis of Active Substances

作　　者：陈倩 张露源 陈伯昌 吴海燕[1] CHEN Qian;ZHANG Lu-yuan;CHEN Bo-chang;WU Hai-yan(Guangxi Key Laboratory of Agric-Environment and Agric-Products Safety,Agricultural College of Guangxi University,Nanning 530004)

机构地区：[1]广西农业环境与农产品安全重点实验室,广西大学农学院,南宁530004

出　　处：《生物技术通报》2021年第7期127-136,共10页Biotechnology Bulletin

基　　金：国家现代农业产业技术体系广西创新团队(nycytxgxcxtd-10-04);广西自然科学基金项目(2020GXNSFDA297003)。

摘　　要：疣孢漆斑菌(Myrothecium verrucaria)ZW-2菌株对大豆孢囊线虫具有(Heterodera glycines)明显的杀线虫活性,为了确定该菌株的最优发酵条件以及代谢产物,采用4因素3水平的正交试验进行了发酵条件优化以及代谢组学分析。结果表明:菌株ZW-2以Czapek培养基为基础培养基,最佳培养温度为28℃,摇床转速为180 r/min,装瓶量为100 mL,培养基pH为8,最佳碳源和氮源为蔗糖和硝酸钠。经代谢组学分析,根据代谢物质荷比、精确分子量及一级碎片离子得分等信息共筛选出20种差异代谢物,包括酰胺类化合物和脂肪族化合物。菌株ZW-2上述试验结果为进一步开发利用该菌株提供重要的理论和应用价值。Myrothecium verrucaria ZW-2 strain had obvious nematicidal activity against Heterodera glycines.In order to determine the optimal fermentation conditions and metabolites of the strain,the orthogonal experiment with four factors and three levels was used to optimize the fermentation conditions and analyze the metabolomics.The results showed that the strain ZW-2 was cultured on Czapek medium with the optimal culture temperature of 28℃,rotation speed of 180 r/min,bottle volume of 100 mL,medium pH of 8,and the optimal carbon and nitrogen sources were sucrose and sodium nitrate.After metabolomics analysis,20 differential metabolites,including amide compounds and aliphatic compounds,were screened according to the information of the charge ratio of metabolites,the accurate molecular weight and the score of first-order fragment ions.The above experiment results by strain ZW-2 further provide important theoretical basis and application value for the development and utilization of this strain.

关 键 词：疣孢漆斑菌 大豆孢囊线虫 发酵条件优化 代谢组学分析 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治] S435.651[农业科学—植物保护]