植物液泡加工酶研究进展  

Advances in Plant Vacuolar Processing Enzymes

作　　者：苏雨 李宗芸 韩永华 SU Yu;LI Zong-yun;HAN Yong-hua(School of Life Sciences,Jiangsu Normal University,Jiangsu Key Laboratory of Phylogenomics&Comparative Genomics,Xuzhou 221116)

机构地区：[1]江苏师范大学生命科学学院,江苏省系统发育与比较基因组学重点实验室,徐州221116

出　　处：《生物技术通报》2021年第6期181-191,共11页Biotechnology Bulletin

基　　金：江苏省研究生科研创新计划(KYCX20_2312);江苏高校优势学科建设工程资助项目(PAPD);现代农业产业技术体系建设专项资金(CARS-10-B3)。

摘　　要：植物液泡加工酶(vacuolar processing enzyme,VPE)是一种天冬氨酸特异性的半胱氨酸蛋白酶,属于Peptidase C13家族,对于天冬氨酸和天冬酰胺残基的羧基肽链有底物专一性。VPE无论是在结构上还是功能上都与动物的caspase-1蛋白具有相似性,VPE是植物中负责液泡蛋白成熟和活化的蛋白酶,在不同的植物器官与植物发育和死亡的不同阶段发挥多功能作用,通过引发液泡破裂和启动蛋白水解级联介导程序性细胞死亡(programmed cell death,PCD)。VPE还具有肽连接活性,它负责在某些植物中产生环肽。被子植物的VPE在进化过程中出现了4种类型:α型VPE、β型VPE、γ型VPE和δ型VPE。总结了植物VPE的进化与分类、成熟和活化过程、VPE的功能,以及该领域的最新进展和未来的研究领域,以便更全面地了解VPE在植物中的作用。Plant vacuolar processing enzyme(VPE)is an aspartic acid specific cysteine protease.VPE belongs to the peptidase C13 family and has substrate specificity for the carboxyl peptide chain of aspartic acid and asparagine residues.VPE is similar to caspase-1 protein from animal in both structure and function.VPE is a protease responsible for maturation and activation of vacuolar proteins in plants.It plays a multifunctional role in different plant organs and different stages of plant development and death,and mediates programmed cell death(PCD)by triggering vacuole rupture and initiating the proteolytic cascade.VPE also has peptide linking activity,which is responsible for the production of cyclic peptides in certain plants.There are four types of VPE in angiosperms during the evolution process:alpha-type VPE,betatype VPE,gamma-type VPE,and delta-type VPE.This article summarizes the evolution,classification,maturation,activation processes and the functions of VPE in plant,as well as the latest progress and future research areas in this field,aiming to more comprehensively understand the role of VPE in plants.

关 键 词：液泡加工酶 程序性细胞死亡 半胱氨酸蛋白酶 生长发育 

分 类 号：Q946.5[生物学—植物学]