高内涵技术检测葛根素对人源肥大心肌细胞BNP表达的影响  

Effect of puerarin on BNP expression in human hypertrophic cardiomyocytes as investigated by high-content screening

作　　者：林育辉[1] 周蕾[1] 戴文军[1] 黄炯华[1] 何晓青[1] Lin Yuhui;Zhou Lei;Dai Wenjun;Huang Jionghua;He Xiaoqing(Department of Cardiology,Third Affiliated Hospital of Guangzhou Medical University,Guangzhou 510150,China)

机构地区：[1]广州医科大学附属第三医院心内科,广东广州510150

出　　处：《广州医科大学学报》2021年第3期28-32,共5页Academic Journal of Guangzhou Medical University

基　　金：广州医科大学大学生实验室开放项目(2018年度);2021年度广东省中医药局科研项目(20202135)。

摘　　要：目的:探讨葛根素对人源肥大心肌细胞脑钠肽(BNP)表达的影响。方法:内皮素-1(ET-1)诱导人源心肌细胞肥大,200μg/mL葛根素对肥大心肌细胞进行预处理及后处理,高内涵仪器MD ImageXpress Nano检测分析肥大心肌细胞BNP的表达量。结果:与对照组相比,肥大心肌细胞BNP表达量增加(P<0.05);与肥大心肌细胞组相比,葛根素预处理及后处理均可降低BNP表达量(P<0.05),其中葛根素预处理降低BNP表达量更为显著(P<0.05)。结论:葛根素对肥大心肌细胞可起到一定的保护作用,为葛根素在心肌肥大治疗方面提供了理论基础。Objective:To investigate the effect of puerarin on the expression of brain natriuretic peptide(BNP)in human hypertrophic cardiomyocytes.Methods:Human hypertrophic cardiomyocytes were induced by endothelin-1(ET-1),and then pre-and post-treated with 200μg/mL puerarin.A high-content analysis system(MD ImageXpress Nano)was used to measure and analyze BNP expression in the hypertrophic cardiomyocytes.Results:Compared with the control group,hypertrophic cardiomyocytes showed increased BNP expression(P<0.05).Compared with untreated hypertrophic cardiomyocytes,those pre-and post-treated with puerarin showed lower expression of BNP(P<0.05).The reduction in BNP expression was greater in hypertrophic cardiomyocytes with puerarin pretreatment(P<0.05).Conclusion:Puerarin exhibits definite protection on hypertrophic cardiomyocytes.This offers a theoretical basis for puerarin in the treatment of cardiac hypertrophy.

关 键 词：葛根素 肥大心肌细胞 脑钠肽 高内涵 

分 类 号：R542.2[医药卫生—心血管疾病]