杜仲U-box基因家族鉴定及分析  

Identification and Analysis of U-box Gene Family in Eucommia ulmoides Oliv.

作　　者：赵丹[1] 李波[1] 赵德刚[1,2] ZHAO Dan;LI Bo;ZHAO Degang(Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education),Institute of Agro-bioengineering,Guizhou University,Guiyang 550025,China;Guizhou Academy of Agricultural Sciences,Guizhou Plant Conservation Technology Center,Guiyang 550006,China)

机构地区：[1]贵州大学农业生物工程研究院/生命科学学院,山地植物资源保护与保护种质创新教育部重点实验室,贵阳550025 [2]贵州省农业科学院,贵州省植物保育技术应用工程研究中心,贵阳550006

出　　处：《种子》2021年第7期10-17,共8页Seed

基　　金：国家自然科学基金项目(31870285);贵州省人才基地项目(RC 2018-16);贵州省高层次创新型人才培养项目(黔科合人才[2016]4003号);中央引导地方科技发展专项资金(黔科中引地[2018]4001)。

摘　　要：植物U-box蛋白是编码含有U-box结构域的基因家族,其编码蛋白大多是泛素系统中特异性识别底物的泛素连接酶E 3,在植物的生长发育、生物及非生物胁迫中起着广泛的调控作用。目前对杜仲的U-box基因了解较少,本研究通过生物信息学和荧光定量PCR技术,对杜仲U-box基因进行鉴定和表达分析。结果表明,杜仲基因组中含有40个U-box基因,根据蛋白结构域将其分为7类,并分析了基因家族成员的基因结构、系统发育、保守结构域和基序等。此外,U-box-Arm结构家族成员的基因表达分析发现,该家族成员的表达具有一定的组织特异性。The U-box genes from plant are a gene family encoding the proteins contained U-box domain,and most of its encoded proteins are ubiquitin-ligase E 3,which can specifically recognize substrates in the ubiquitin system,and play a wide range of regulatory roles in plant growth and development as well as biotic and abiotic stresses.At present,the U-box genes of Eucommia ulmoides are poorly understood.In this study,bioinformatics and real-time PCR were used to identify and analyze the expression of E.ulmoides U-box genes.The results showed that there were 40 U-box genes in E.ulmoides genome,which were divided into 7 classes according to the protein domain.The gene structure,phylogeny,conserved domain and motif of gene family members were also analyzed.In addition,gene expression analysis of the members of gene family contained U-box-ARM domain revealed that the expression of the members of this family had a certain tissue specificity.

关 键 词：杜仲 U-box基因家族 生物信息学分析 表达分析 

分 类 号：S567.19[农业科学—中草药栽培]