血管生成性T淋巴细胞对子痫前期大鼠胎盘灌注的影响  

Improvement of Placental Perfusion in Preeclampsia Model Rat by Transplantation of Angiogenic T Cells

作　　者：曹武占 郭叶青[1] 贺鑫[1] 刘佳[1] 邓利 陆佳黛 姚穗[1] CAO Wu-zhan;GUO Ye-qing;HE Xin(Department of Obstetrics,Hunan Provincial Peoples Hospital,Changsha 410005,China)

机构地区：[1]湖南师范大学附属第一医院/湖南省人民医院产科,湖南长沙410005

出　　处：《医学临床研究》2021年第7期1048-1051,共4页Journal of Clinical Research

摘　　要：[目的]探讨血管生成性T淋巴细胞(angiogenic T cells,Tang)对子痫前期大鼠胎盘灌注的影响。[方法]采用密度梯度离心法分离健康孕妇胎盘脐带血的单个核细胞,经磁珠分选(MACS)和流式细胞仪分选Tang,再进行培养,制备细胞悬液。选取30只成年健康雌性大鼠,发情期按2:1与雄性大鼠同笼饲养,每日观察阴栓脱落情况,发现阴栓日为妊娠d。30只妊娠大鼠随机分为实验组、对照组和正常组,每组10只。实验组和对照组孕鼠自妊娠dr连续5d腹腔注射左旋精氨酸甲酯(L-NAME)250 mg/(kg·d),分别于妊娠d、d1o、d13、dio、dyg测量大鼠尾动脉收缩压,发现血压明显升高视为子痫前期孕鼠模型建模成功。建模成功后实验组在尾静脉注射Tang,对照组在尾静脉注射等量生理盐水,均连续6 d。正常组未做任何处理。比较三组大鼠血压、尿蛋白浓度、胎盘血管密度、胎盘重量、胎鼠体重等指标。[结果]妊娠di3、dio、dy,实验组和对照组孕鼠血压、尿蛋白浓度均高于正常组;妊娠dno、dg,实验组血压和尿蛋白浓度显著低于对照组,其差异均有统计学意义(P<0.05)。三组胎鼠数、胎盘重量比较差异无统计学意义(P>0.05);实验组和对照组胎鼠出生体重、胎盘新生血管荧光密度均显著低于正常组,但实验组胎鼠出生体重、胎盘新生血管荧光密度显著高于对照组,其差异均有统计学意义(P<0.05)。胎盘HE染色显示:对照组孕鼠胎盘组织细胞排列紊乱,细胞量少,细胞水肿和空泡样改变较多,胎盘血管间膜增厚,可见纤维素样坏死;实验组胎盘组织较对照组明显改善,无明显细胞水肿及空泡,仍有少量纤维素样坏死,新生血管较对照组明显增多。[结论]Tang移植在一定程度上增加子痫前期大鼠胎盘新生血管密度和改善妊娠结局,表明对胎盘灌注有一定的改善作用。[Objective]The purpose was to investigate whether the placental perfusion in preeclampsia model rats can be improved by transplantation of angiogenic T cells(Tang).[Methods]Thirty adult healthy female rats were selected and kept in the same cage as the male rats at a ratio of 2:1 during the estrus period.The vaginal plug shedding was observed daily;and it was marked as pregnancy dl when the day of the vaginal plug was seen.Then,30 pregnant rats were randomly divided into experimental group,control group and normal group,with 10 rats in each group.The rats model of preeclampsia was induced by intraperitoneal infected with L-NAME.The pregnant rats of the experimental group and the control group were intraperitoneally injected with L.arginine methyl ester(L-NAME)250 mg/(kg·d)from d;of pregnancy for 5 consecutive days.and the tail arteries of rats were measured on d,do.ds,d6,and dio of pregnancy,respectively.The systolic blood pressure is found to be significantly elevated,which is regarded as the successful modeling of the preeclampsia pregnant mouse model.Umbilical venous blood was taken from healthy pregnant rats,then isolation of cord blood mononuclear cells was performed by density gradient centrifugation,followed by magnetic activated cell sorting(MACS)and flow cytometry for the isolation Tang cells.The Tang cells were transplanted by means of tail vein injection to the rats with preeclampsia.Meanwhile,the control group was injected with the same amount of normal saline in the tail vein for 6 consecutive days.No treatment was done in the normal group.T he blood pressure,urine protein concentration,placental blood vessel density,placental weight,fetal weight and other indicators were compared among the three groups.The micro vascular density of placenta was detected by positive expression of CD31 as shown by inmunofluorescence histochemistry.The gestational result was observed by measurement of the weight of placenta rat fetus and placenta.[Results]On dis,do,and ds of pregnancy,the blood pressure and urine prot

关 键 词：妊娠 动物 先兆子痫 大鼠 新生血管化 病理性 T淋巴细胞 胎盘血液循环 

分 类 号：R714.245[医药卫生—妇产科学]