Piperlongumine Inhibits Zika Virus Replication In vitro and Promotes Up-Regulation of HO-1 Expression, Suggesting An Implication of Oxidative Stress  

作　　者：Weizhi Lu Linjuan Shi Jing Gao Huimin Zhu Ying Hua Jintai Cai Xianbo Wu Chengsong Wan Wei Zhao Bao Zhang 

机构地区：[1]Biosafety Level-3 Lab,School of Public Health,Southern Medical University,Guangzhou 510515,China [2]Department of Epidemiology,School of Public Health,Southern Medical University,Guangzhou 510515,China

出　　处：《Virologica Sinica》2021年第3期510-520,共11页中国病毒学（英文版）

基　　金：supported by the National Natural Science Foundation (Nos. 31670168, 31470271 and 81730110);National Key R&D Program of China (Grant No. 2018YFC1602206);Guangdong Provincial Science and Technology (No. 2018B020207006)。

摘　　要：Owing to the widespread distribution of mosquitoes capable of transmitting Zika virus, lack of clinical vaccines and treatments, and poor immunity of populations to new infectious diseases, Zika virus has become a global public health concern. Recent studies have found that Zika virus can continuously infect human brain microvascular endothelial cells.These cells are the primary components of the blood–brain barrier of the cerebral cortex, and further infection of brain tissue may cause severe damage such as encephalitis and fetal pituitary disease. The present study found that a biologically active base, piperlongumine(PL), inhibited Zika virus replication in human brain microvascular endothelial cells, Vero cells, and human umbilical vein endothelial cells. PL also significantly increased heme oxygenase-1(HO-1) gene expression, while silencing HO-1 expression and using the reactive oxygen species scavenger, N-acetylcysteine, attenuated the inhibitory effect of PL on Zika virus replication. These results suggest that PL induces oxidative stress in cells by increasing reactive oxygen species. This, in turn, induces an increase in HO-1 expression, thereby inhibiting Zika virus replication. These findings provide novel clues for drug research on the prevention and treatment of Zika virus.

关 键 词：Piperlongumine Heme oxygenase-1(HO-1) Reactive oxygen species Zika virus(ZIKV) Human brain microvascular endothelial cells 

分 类 号：R373[医药卫生—病原生物学]