CRISPR/Cas9系统在稻米品质改良中的应用  

Applications of the CRISPR/Cas9 System for Rice Grain Quality Improvement

作　　者：冷春旭[1,2,3] 闫平 吴立成[1,2,3] 王玉杰 Leng Chunxu;Yan Ping;Wu Licheng;Wang Yujie(Biotechnology Research Institute of Heilongjiang Academy of Agricultural Sciences,Heilongjiang,150028;Northern Japonica Rice Molecular Breeding Joint Research Center,Chinese Academy of Sciences,Heilongjiang,150028;Key Laboratory of Crop and Livestock Molecular Breeding of Heilongjiang,Biotechnology Research Institute of Heilongjiang Academy of Agricultural Sciences,Heilongjiang,150086)

机构地区：[1]黑龙江省农业科学院生物技术研究所,黑龙江150028 [2]中国科学院北方粳稻分子育种联合研究中心,黑龙江150028 [3]黑龙江省农业科学院生物技术研究所,黑龙江省作物与家畜分子育种重点实验室,黑龙江150086

出　　处：《分子植物育种》2021年第14期4681-4689,共9页Molecular Plant Breeding

基　　金：国家重点研发计划(2017YFD0100504);国家现代农业产业技术体系(CARS-01-54);农业科研杰出人才培养计划(2016-2020-48);国家粮食丰产工程项目(2017YDF-0300506-6);黑龙江省农业科学院院级科研项目(2019YYYF011)共同资助。

摘　　要：目前稻米品质改良已成为水稻育种的重要目标。稻米品质主要包括外观、加工、蒸煮食味和营养品质,由极其复杂的基因调控网络决定。CRISPR/Cas9系统作为新发展的定向基因编辑系统为水稻品质改良提供了重要的技术支撑,该系统具有操作简单、编辑效率高、易于多基因编辑等优点。本研究结合CRISPR/Cas9基因编辑系统及稻米品质相关基因的研究进展,详细介绍了CRISPR/Cas9基因编辑系统在稻米品种改良中的应用,讨论了该技术在水稻品质改良中存在的问题和改进策略,旨在为利用CRISPR/Cas9技术创制优质水稻新品种提供参考。At present,grain quality improvement has become a key target for rice breeding.Rice quality mainly includes appearance,processing,cooking taste and nutritional quality,which is determined by the extremely complicated gene regulation network.As a newly developed targeted gene-editing system,the CRISPR/Cas9 system provides important technical support for rice quality improvement.The system has the advantage of simple operation,high editing efficiency,and easy multi-gene editing.In this study,the combines the research progress of the CRISPR/Cas9 gene-editing system and rice quality-related genes,then introduces in detail the application of the CRISPR/Cas9 system in rice variety improvement.Finally,the problems and improvement strategies of this technology were discussed,the purpose is to provide a reference for creating new high-quality rice varieties using CRISPR/Cas9 technology.

关 键 词：CRISRP/Cas9 基因编辑 品质改良 水稻 

分 类 号：S511[农业科学—作物学]