利用重组自交系定位水稻种子耐低温发芽QTL  

QTL Involved in Low-temperature Tolerance at the Germination Stage by Recombination Inbred Lines in Rice

作　　者：张所兵[1] 张云辉[1] 林静[1] 陈海元 汪迎节[1] 朱晓妹 宋春凤[2] 方先文[1] Zhang Suobing;Zhang Yunhui;Lin Jing;Chen Haiyuan;Wang Yingjie;Zhu Xiaomei;Song Chunfeng;Fang Xianwen(The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm,Institute of Crop Germplasm and Biotechnology,Jiangsu Academy of Agricultural Sciences,Nanjing,210014;Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing,210014)

机构地区：[1]江苏省农业科学院种质资源与生物技术研究所,江苏省农业种质资源保护与利用平台,南京210014 [2]江苏省中国科学院植物研究所,南京210014

出　　处：《分子植物育种》2021年第14期4690-4695,共6页Molecular Plant Breeding

基　　金：江苏省农业科技自主创新资金(CX〔18〕1001);2018年中医药公共卫生服务补助专项(财社〔2018〕43号)共同资助。

摘　　要：具有强耐低温发芽直播稻的种子可以克服低温胁迫导致的发育迟缓,保证幼苗旺盛生长。水稻种子耐低温发芽是多基因控制的复杂数量性状,遗传力低,导致育种家对该性状直接选择的效率很低。耐低温发芽QTL定位研究,有助于开展分子标记辅助选择,提高选择效率。本研究发现,江苏省优质粳稻品种‘南粳46’与云南地方品种‘扎西玛’低温发芽率存在极显著差异。利用‘扎西玛’‘/南粳46’RIL群体定位了3个控制低温发芽的QTL(qLTG-2,qLTG-4和qLTG-7),分别位于第2、4和7染色体上。3个QTL分别可以解释7.69%、8.75%和22.93%表型变异。其中,qLTG-2所在的染色体区域未见报道QTL,是新的QTL位点。该结果为耐低温发芽分子标记辅助选择育种提供了材料基础和分子标记。Low-temperature germination resistance of direct-seeded rice seeds can overcome the developmental delay caused by low-temperature stress and ensure the vigorous growth of seedlings.The low-temperature tolerance at germination stage is complex quantitative trait controlled by QTLs and has low genetic force,which leads to low efficiency of the direct selection of the trait by breeders.The research on QTL mapping for lowtemperature tolerance at germination stage is helpful to carry out molecular marker-assisted selection and improve the efficiency of selection for low-temperature germination at tolerance stage in direct-seeded rice breeding.In this study,the low-temperature germination rate showed extremely significant differences between the high-quality japonica rice variety'Nanjing46'in Jiangsu and the local variety'Zhaxima'in Yunnan.The QTL mapping was carried out by recombination inbred lines derived from a cross between'Zhaxima'and the'Nanjing46'.Total three QTLs(qLTG-2,qLTG-4 and qLTG-7)involved in low-temperature tolerance at the germination stage were detected on chromosome 2,4 and 7,respectively.They accounted for 7.69%,8.75%and 22.93%of the total phenotypic variation,respectively.Interestingly,there was no reported QTL involved in low-temperature tolerance at the germination stage in the candidate region of qLTG-2,which exhibited that the qLTG-2 was a novel QTL locus.The QTLs detected in this study will provide new genetic material and molecular markers for improving the low-temperature tolerance at germination stage in rice by molecular marker assisted selection breeding.

关 键 词：直播稻 重组自交系 低温发芽 QTL定位 

分 类 号：S511[农业科学—作物学]