玉米穗位叶叶绿素含量的QTL定位分析  

QTL Mapping for Chlorophyll Content in Maize Ear Leaf

作　　者：肖仁杰 柳志华 胡锦祥 朱雪晴 李芳 徐莹 罗红兵[1,2] 黄成 Xiao Renjie;Liu Zhihua;Hu Jinxiang;Zhu Xueqing;Li Fang;Xu Ying;Luo Hongbing;Huang Cheng(College of Agronomy,Hunan Agricultural University,Changsha,410128;Maize Engineering Technology Research Center of Hunan Province,Changsha,410128;Hunan Provincial Key Laboratory for Germplasm Innovation and Utilization of Crop,Hunan Agricultural University,Changsha,410128)

机构地区：[1]湖南农业大学农学院,长沙410128 [2]湖南省玉米工程技术研究中心,长沙410128 [3]湖南农业大学,湖南省作物种质创新与资源利用重点实验室,长沙410128

出　　处：《分子植物育种》2021年第14期4703-4707,共5页Molecular Plant Breeding

基　　金：湖南省自然科学基金项目(2020JJ5235);作物种质创新与资源利用重点实验室开放研究项目(19KFXM10);湖南省教育厅优秀青年项目(18B117);湖南农业大学青年科学基金项目(19QN16)共同资助。

摘　　要：叶绿素含量是决定作物光合效率的重要因素,也是作物高光效育种的重要目标性状之一。本研究利用玉米自交系W22与玉米野生祖先种大刍草杂交衍生得到的包含866个家系的渗入系群体,结合均匀覆盖玉米全基因组的19838个SNP分子标记,采用R/qtl的多QTL模型对玉米开花期穗位叶的叶绿素含量进行高精度的QTL定位分析。结果共检测到10个控制穗位叶叶绿素含量的QTL,分别位于第1、3、4、5、6、7、8和9染色体,单个QTL表型贡献率的变幅为2.02%~4.04%,加性效应的变幅为1.61~3.03,表明玉米穗位叶叶绿素含量属于典型的数量性状,由微效多基因控制。本研究可以为进一步解析玉米穗位叶叶绿素含量的遗传基础、图位克隆相关关键基因及分子标记辅助选择育种改良玉米植株叶绿素含量提供了重要的参考价值。Chlorophyll content is an important factor that determines the photosynthetic efficiency of crops,and it is also one of the important target traits of high photosynthetic efficiency breeding.In this study,we performed a high-resolution QTL mapping for chlorophyll content in maize ear leaf using a large population of 866 maize-teosinte introgression lines that has been genotyped with 19838 SNP markers.The results showed that a total of 10 QTLs for chlorophyll content in maize ear leaf were located on chromosome 1,3,4,5,6,7,8 and 9.The phenotypic contribution rate and additive effect of each QTL ranged from 2.02%to 4.04%and from 1.61 to 3.03,respectively.These results indicated that the chlorophyll content in maize ear leaf is a typical quantitative trait,which is controlled by multiple micro-effect genes.This study might provide an important reference value for elucidating the genetic basis of chlorophyll content in maize ear leaf,cloning related key genes and improving chlorophyll content of maize plants using molecular marker-assisted selection.

关 键 词：玉米 穗位叶 叶绿素含量 QTL定位 

分 类 号：S513[农业科学—作物学]