硅对干旱胁迫下燕麦根系响应的分子机理  

Molecular Mechanism of Silicon Response to Oat Root under Drought Stress

作　　者：张杰 尹强[1] 闫志坚[1] 万永青[2] 王育青[1] Zhang Jie;Yin Qiang;Yan Zhijian;Wan Yongqing;Wang Yuqing(Institute of Grassland Research of Chinese Academy Agricultural Sciences,Hohhot,010010;Inner Mongolia Key Laboratory of Plant Stress Physiology and Molecular Biology,College of Life Sciences,Inner Mongolia Agricultural University,Hohhot,010010)

机构地区：[1]中国农业科学院草原研究所,呼和浩特010010 [2]内蒙古农业大学生命科学学院,内蒙古自治区植物逆境生理与分子生物学重点实验室,呼和浩特010010

出　　处：《分子植物育种》2021年第14期4759-4769,共11页Molecular Plant Breeding

基　　金：国家牧草产业技术体系鄂尔多斯综合试验站(CARS-34);国家重点研发计划课题(2016YFC0500605);中央级公益性科研院所基本科研业务费专项资金(中国农业科学院草原研究所1610332018002)共同资助。

摘　　要：硅对非生物胁迫下植物生长发育的帮助已有许多报道,但硅在干旱胁迫下影响燕麦根系的分子机制尚不清楚。本试验采用加拿大引进燕麦品种‘甜燕Ⅰ号’,以Na2SiO3·9H2O作为硅源,以聚乙二醇(PEG-6000)模拟干旱胁迫环境,研究了对照处理、干旱胁迫处理(25%PEG-6000)和硅+干旱胁迫处理(10 mmol/L Na2SiO3·9H2O+25%PEG-6000)条件下对燕麦根系的影响,通过转录组分析(176555个基因),旨在比较3种处理条件下燕麦根系的差异表达基因。比较转录组学分析表明,在干旱胁迫下,硅在改变234个基因的表达水平中起着重要作用。GO富集分析表明,氧化还原酶活性、细胞氮化合物代谢过程、细胞大分子代谢过程等相关基因的富集程度显著提高,并且这些基因与非生物抗性有着密切的联系,表明硅可能诱导燕麦幼苗的耐旱性。通过研究硅介导的抗旱机制,为干旱地区农作物生产中硅的应用提供理论依据。There are many reports that silicon has beneficial effects on plant growth and development under abiotic stress,the molecular mechanism of silicon affecting oats under drought stress is unclear.This test variety was introduced from Canada,Sweety.Na2SiO3·9H2O was used as the silicon source,and polyethylene glycol(PEG-6000)was used to simulate the drought stress environment.Normal growth as control,drought stress(25%PEG-6000),and silicon+drought stress(10 mmol/L Na2SiO3·9H2O+25%PEG-6000)on the root of oats were analyzed by transcriptome analysis(176555 single genes)in order to compare the differential genes of plants under the three treatments.Comparative transcriptomics analysis showed that silicon plays an important role in changing the expression levels of 234 genes under drought stress.GO enrichment analysis showed that the enrichment of related genes such as oxidoreductase activity,cellular nitrogen compound metabolism,and cellular macromolecular metabolism significantly increased,and these genes are inextricably linked to abiotic resistance,indicating that silicon may induce drought tolerance in oat seedlings.By studying the mechanism of silicon-mediated drought resistance,it provides a theoretical basis for the application of silicon in crop production in arid regions.

关 键 词：燕麦 干旱胁迫 硅 转录组 

分 类 号：S512.6[农业科学—作物学]