Taz及Runx2在大鼠下颌第一磨牙发育中的表达研究  

The expression of Taz and Runx2 in the first mandibular molar of developing rat

作　　者：张冰 徐欣 ZHANG Bing;XU Xin(Department of VIP Center,School and Hospital of Stomatology,Cheeloo College of Medicine,Shandong University Shandong Key Liboratory of Oral Tissue Regeneration,Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration,Jinan 250012,China)

机构地区：[1]山东大学齐鲁医学院口腔医学院特诊科口腔医院特诊科&山东省口腔组织再生重点实验室山东省口腔生物材料与组织再生工程实验室,250012 [2]山东大学齐鲁医学院口腔医学院种植科口腔医院特诊科&山东省口腔组织再生重点实验室山东省口腔生物材料与组织再生工程实验室,250012

出　　处：《口腔医学》2021年第7期583-587,共5页Stomatology

基　　金：国家自然科学基金(82071148)。

摘　　要：目的观察具有PDZ结合基序的转录辅激活物(transcriptional coactivator with PDZ-binding motif,Taz)、Runt相关转录因子2(Runt-related transcription factor 2,Runx2)在大鼠下颌第一磨牙发育过程中的表达,分析Taz在牙齿发育中的作用及二者相互关系。方法获取牙胚各发育时期的标本,通过免疫组织化学的方法观察Taz、Runx2的表达与定位。结果Taz在蕾状期、帽状期及钟状早期的口腔上皮及间充质中均有阳性表达。随着牙胚的发育,Taz在钟状早期时表达最强,可见在内釉上皮层、外釉上皮层、中间层及牙囊中均呈现Taz的强阳性表达。Taz在出生后的成牙本质细胞及前期牙本质中的表达呈阳性,在成釉细胞中的表达随着釉质的矿化而逐渐减弱。Runx2在牙胚早期发育的各时期具有表达的特异性,在蕾状期及帽状期可见Runx2在口腔上皮及间充质中的阳性着色,在钟状早期Runx2除了口腔上皮及间充质的阳性表达外,在内釉上皮层、中间层、牙乳头及牙囊中也呈现阳性的表达;在出生后分泌期的成牙本质细胞中Runx2呈阳性表达,随着成牙本质细胞的分化表达逐渐降低。结论Taz在大鼠下颌第一磨牙发育中呈现特异性表达,推测Taz可能是牙齿发育过程中一个重要的调控因子;Taz与Runx2的表达与定位相似,二者在调控牙齿发育中可能存在一定的相关性。Objective To investigate the spatial and temporal expression of transcriptional coactivator with PDZ-binding motif(Taz) and Runt-related transcription factor 2(Runx2) during the development of first molar in rat mandibular and analyze the function of Taz in tooth development and the interrelation between Taz and Runx2. Methods Samples were collected from Wistar rat at various stages. Immunohistochemical method was used to observe the expression pattern and localization of Taz and Runx2. Results At the bud stage, cap stage and early bell stage, the positive expression of Taz was detected in the oral epithelium and dental mesenchyme. With the development of tooth germ, the strongest staining of Taz appeared in early bell stage. Strongly positive expression of Taz was found in the inner enamel epithelium, outer enamel epithelium, stratum intermedium and dental follicle. The expression of Taz was positive in postnatal odontoblasts and predentin, and its expression level in ameloblasts decreased with the mineralization of enamel. Runx2 in different stages presented different expression and localization in embryonic samples. At the bud and cap stage, Runx2 was found in the oral epithelium and dental mesenchyme. At the early bell stage, positive staining was detected in the inner enamel epithelium, stratum intermedium, dental papilla and dental follicle, in addition to oral epithelium and dental mesenchyme. Runx2 expression in odontoblasts which was in secretory phase was positive and the staining gradually reduced with the differentiation of odontoblasts. Conclusion The expression of Taz during the development of first molars in rat mandibular is spatiotemporal. The result suggests that Taz may be an important regulatory factor in tooth development. The expression and localization of Taz was similar to Runx2, indicating that there is certain correlation between Taz and Runx2 in regulating tooth development of rat.

关 键 词：PDZ结合基序的转录辅激活物 Runt相关转录因子2 牙齿发育 免疫组织化学 

分 类 号：R392.114[医药卫生—免疫学]