电针对慢性脑缺血大鼠学习记忆及海马神经干细胞分化的影响  

The effects of electro-acupuncture on learning,memory and stem cell differentiation in the hippocampus of rats modeling chronic cerebral ischemia

作　　者：魏居瑞 艾琪 刘佩[2] 夏玮 钱巍 蒋松涛 郑君霞 朱松杰 陈赟 Wei Jurui;Ai Qi;Liu Pei;Xia Wei;Qian Wei;Jiang Songtao;Zheng Junxia;Zhu Songjie;Chen Yun(Rehabilitation Department,The First People′s Hospital of Lin′an District,Linan 311300,China;Department of Acupuncture and Moxibustion,The Third People′s Hospital of Haining City,Haining 314400,China;Department of Pain Medicne,Zhengzhou Hospital of Traditional Chinese Medicine,Zhengzhou 450000,China)

机构地区：[1]浙江省临安区第一人民医院康复科,临安311300 [2]浙江省海宁市第三人民医院针灸科,海宁314400 [3]郑州市中医院疼痛科,郑州450000

出　　处：《中华物理医学与康复杂志》2021年第7期582-587,共6页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：浙江省自然科学基金项目(Y205389);浙江省自然科学基金项目(Y2101356);杭州市临安区科技局项目(临科字2020第31号)。

摘　　要：目的观察电针对慢性脑缺血大鼠学习记忆能力和海马神经干细胞分化的影响。方法选取雄性Sprague Dawley大鼠120只,采用改良永久性结扎双侧颈总动脉法造成慢性脑缺血模型,将造模成功的104只大鼠分为模型组和电针组,每组52只,电针组予以电针治疗,输出电流1 mA,频率15 Hz,连续波,每次20 min,每日1次,治疗7次后休息2 d。模型组不予特殊处理。按照治疗时间的不同,将每组大鼠再细分为术后1、2、4、6周4个亚组,每个亚组13只。术后1、2、4、6周,每组随机抽取6只大鼠进行BrdU注射,观察神经干细胞增殖及分化情况。利用Morris水迷宫、BrdU+NeuN及BrdU+GFAP免疫荧光双标方法,分别观察大鼠的空间学习能力及记忆能力,探讨海马齿状回神经发生的规律。结果电针组大鼠在术后2、4、6周的学习记忆能力明显高于模型组大鼠(P<0.05);术后2周,大鼠海马颗粒细胞层细胞出现BrdU+NeuN及BrdU+GFAP双阳性表达,电针组大鼠分化神经元的数量多于模型组(P<0.05)。与模型组同时间点比较,电针组术后4周的GFAP阳性细胞数少,术后6周的GFAP阳性细胞数多,但差异均无统计学意义(P>0.05)。结论电针可以促进神经干细胞的分化,进而改善慢性脑缺血大鼠的学习记忆能力。Objective To observe the effect of electro-acupuncture(EA)on learning,memory and the differentiation of neural stem cells in the hippocampus in the presence of chronic cerebral ischemia.Methods A total of 120 male Sprague-Dawley rats had chronic cerebral ischemia induced by bilateral ligation of the common carotid arteries.The model was successfully established in 104 of them,and they were randomly divided into a model group and an EA group,each of 52.The EA group was given 20 minutes of EA at acpoints Baihui and Dahui every day for 7 days,followed by an interval of 2 days.The current output was 1mA and the frequency was 15Hz.The model group was not given any intervention.One,2,4 and 6 weeks after the modelling,6 rats from each group were injected with BrdU,and any proliferation and differentiation of neural stem cells was observed.The rats′learning and memory were also evaluated using the Morris water maze,and neurogenesis of in the dentate gyrus was observed using BrdU+NeuN and BrdU+GFAP double-labelled immunofluorescence.Results The learning and memory of the EA group were significantly better than those of the model group 2,4 and 6 weeks after the modelling.After two weeks BrdU+NeuN and BrdU+GFAP-positive cells were found in the granule cell layer of the hippocampus,and compared with the model group,there were siginificantly more such neurons in the EA group.The gliocyte levels were not significantly different.Conclusion Electro-acupuncture can improve learning and memory in the face of chronic cerebral ischemia by promoting the differentiation of neural stem cells.

关 键 词：电针 脑缺血 大鼠 神经干细胞 分化 

分 类 号：R245[医药卫生—针灸推拿学]