TetR家族转录调控子基因敲除对鲍曼不动杆菌生物被膜生成能力的影响  

Infl uence of Knocking out of TetR Family Transcriptional Regulator Gene on Biofi lm Formation Ability in Acinetobacter baumannii

作　　者：张彦鹏[1] 陶志远 罗勇[1] 丁芳林[1] 樊冰[1] ZHANG Yan-peng;TAO Zhi-yuan;LUO Yong;DING Fang-lin;FAN Bing※(Shenzhen Second People’s Hospital,Guangdong Shenzhen 518035)

机构地区：[1]深圳市第二人民医院,广东深圳518035

出　　处：《深圳中西医结合杂志》2021年第11期1-4,F0003,共5页Shenzhen Journal of Integrated Traditional Chinese and Western Medicine

基　　金：深圳市科技计划基础研究面上项目资助课题(JCYJ20190806164011195)。

摘　　要：目的:研究TetR家族转录调控子(TFR)基因敲除对鲍曼不动杆菌生物被膜(BF)生成能力的影响。方法:通过构建自杀质粒的方法敲除鲍曼不动杆菌SZ042株TFR基因,并设计内部引物和外部引物的方法进行敲除株的验证,采用结晶紫染色法对比观察和定量分析基因敲除前后BF生成的情况。结果:成功敲除鲍曼不动杆菌中TFR基因,获得TFR基因敲除株SZ042/ΔtetRt株;与野生株相比,敲除株菌膜形成量明显下降,培养4、8、12、16、24 h BF的生成量差异均具有统计学意义(P<0.05)。结论:TFR基因对鲍曼不动杆菌被膜生成和形态维持有促进作用,可以作为开发针对鲍曼不动杆菌BF生成机制的新型抗菌药物的潜在靶点。Objective To study the infl uence of knocking out TetR family transcriptional regulator(TFR)gene on the biofi lm formation ability of Acinetobacter baumannii.Methods The TFR gene of Acinetobacter baumannii SZ042 strain was knocked out by constructing suicide plasmid,and the result of knocking was verifi ed through polymerase chain reaction method.Crystal violet staining was used to observe and quantitatively analyze the biofi lm formation before and after the knocking test.Results The TFR gene of Acinetobacter baumannii was knocked out successfully.Compared with the wild strain,the amount of biofi lm formation of SZ042/ΔtetRt strain was signifi cantly decreased,The difference in the amount of BF produced during 4,8,12,16,and 24 hours of culture was statistically signifi cant(P<0.05).Conclusions TFR gene might promote the formation and morphology of biofi lm of Acinetobacter baumannii,and might be a potential target for the development of new antimicrobial agents for Acinetobacter baumannii infection.

关 键 词：鲍曼不动杆菌 生物被膜 TetR家族转录调控子基因 基因敲除 

分 类 号：R378[医药卫生—病原生物学]