儿童供者单个核细胞采集效率的多因素分析  

Multivariate analysis of peripheral blood mononuclear cells from children donors

作　　者：高旖鑫 莫淑宜 王刚 何苏英 李少文[1] 郭楚霞 陆志刚[1] 彭永正[1] GAO Yixin;MO Shuyi;WANG Gang;HE Suying;LI Shaowen;GUO Chuxia;LU Zhigang;PENG Yongzheng(Department of Transfusion Medicine,Zhujiang Hospital of Southern Medical University,Guangzhou 510282,China;Zhaoqing First Peopled Hospital)

机构地区：[1]南方医科大学珠江医院输血科,广东广州510280 [2]肇庆市第一人民医院

出　　处：《中国输血杂志》2021年第7期688-691,共4页Chinese Journal of Blood Transfusion

摘　　要：目的分析健康儿童外周血单个核细胞(PBMC)的采集特点,探讨影响采集效率(collection efficiency, CE)的因素。方法回顾性分析2017年1月至2020年6月42例儿童均在南方医科大学珠江医院进行采集,应用血细胞分离机进行70次PBMC采集的临床资料。结果多重线性回归分析显示,健康儿童供者采集物中单个核细胞(MNC)与抗凝剂用量、淋巴细胞计数、单核细胞计数呈正相关(R^(2)=0.879,P均<0.05),与年龄、血小板计数呈负相关。采集物PBMC效率(CE)与年龄、血小板计数、处理的全血容量呈负相关(r=-0.696,P均<0.05)。CD34^(+)细胞绝对值(×10^(7)/L)与年龄呈负相关,与采集次数、处理的全血容量呈正相关(r=-0.79)。健康儿童供者PBMC的采集效果与采集前后红细胞计数(RBC)、血小板计数(PLT)、淋巴细胞计数(Lymph)、单核细胞计数(Mono)无统计学差异。结论不同年龄的儿童均可有效的采集PBMC。单个核细胞采集效率与儿童年龄相关。采集前淋巴细胞、采集前单核细胞量越多,采集到的MNC量越多。儿童单采>1倍全血总容量后,采集PBMC效率下降,但最终采集物CD34^(+)细胞绝对值上升。Objective To analyze the characteristics of peripheral blood mononuclear cells(PBMC)collection in healthy children,and explore the factors affecting collection efficiency(CE).Methods The PBMC data,involving 70 episodes of apheresis from 42 children during January 2017 and June 2020 were retrospectively analyzed All children were collected in Zhujiang Hospital of Southern Medical University.Results Multiple linear regression analysis showed that mononuclear cells(MNC)in donor collections from healthy children were positively correlated with anticoagulant dosage,lymphocyte count and monocyte count(P<0.05),meanwhile,negatively correlated with age and platelet count.The PBMC CE was negatively correlated with age,platelet count,and processed whole blood volume(P<0.05).CD34^(+)cells(×10^(7)/kg)was negatively correlated with age,meanwhile,positively correlated with numbers of collection and processed whole blood volume(r=-0.79).No statistical differences in red blood cell count,platelet count,lymphocyte count,monocyte count of healthy child donors were notable before versus after apheresis.Conclusion MNC can be collected effectively in children of different ages.The PBMC collection efficiency was related to age.Meanwhile,the higher the lymphocytes and monocytes were before apheresis,the more MNC were collected.The efficiency of MNC collection would decrease when the apheresis volume of the children exceeded their total blood volume twice.However,the absolute value of CD34+cells in the final yields would increase.

关 键 词：血细胞分离机 儿童 外周血单个核细胞 造血干细胞采集 

分 类 号：R551[医药卫生—血液循环系统疾病] R457.1[医药卫生—内科学]