Cromer血型系统抗-IFC的鉴定及特征分析——附1例报告  

Identification and characterization of Cromer blood group anti-IFC

作　　者：莫春妍[1] 魏玲[1] 王贞[1] 廖志坚[1] 姬艳丽[1] 张润青[1] 罗广平[1] MO Chunyan;WEI Ling;WANG Zhen;LIAO Zhijian;JI Yanli;ZHANG Runqing;LUO Guangping(Institute of Clinical Transfusion,Guangzhou Blood Center,Guangzhou 510095,China)

机构地区：[1]广州血液中心广州市血液安全重点实验室,广东广州510095

出　　处：《中国输血杂志》2021年第7期764-767,共4页Chinese Journal of Blood Transfusion

基　　金：广州市医学重点学科建设项目(血液安全重点实验室)。

摘　　要：目的探讨Cromer血型系统抗体抗-IFC的鉴定方法并分析其特性。方法使用试管法鉴定患者ABO血型,使用Rh分型卡鉴定患者Rh血型;使用抗球微柱凝集法对患者血清进行不规则抗体筛选及抗体鉴定;患者血清分别与用抗胰蛋白酶、胰酶和菠萝酶处理的谱细胞进行反应,初步判定其抗体的特异性;用CD55重组蛋白对患者血清进行中和试验,确定其特异性;对Cromer血型系统调控基因DAF进行测序;用流式细胞术对患者红细胞表面CD55抗原表达量进行检测。结果患者血型为B型,CcDEe;患者血清与未经酶处理谱细胞反应凝集强度均为4+;与胰酶和菠萝酶处理后的谱细胞均为4+;与二硫苏糖醇处理谱细胞反应强度减弱至2+^(s);与抗胰蛋白酶处理后谱细胞反应为阴性,其血清中的抗体可被CD55重组蛋白中和,DAF测序无发现突变位点;流式显示该患者CD55抗原存在缺失。结论该抗体为Cromer血型系统高频抗体抗-IFC,考虑为患者在患病期间因消化道疾病因素导致Cromer血型抗原减弱。Objective To explore the identification method and characteristics of anti-IFC against Cromer blood group.Methods ABO blood group was identified by tube method,and Rh blood group was identified by Rh typing card.Irregular antibody screening and antibody identification were carried out using microtube column agglutination technology(MCAT).The serum of the patient reacted with panel cells treated with antitrypsin,trypsin and bromelain respectively to determine the specificity of the antibody.The serum was inhibited with pure CD55 protein for antibody identification.The DAF,the regulatory gene of Cromer blood group system,was amplified and sequenced.The expression of CD55 on cell membrane was analyzed by flow cytometry.Results The patient′s blood type was B,CcDEe.The patient′s serum reacted with all the untreated panel red cells,bromelain-treated red cells,trpsin-treated red cells,and DTT treated red cells.It was negative with chymotypsin-treated cells and could be neutralized by CD55 protein.No mutation was found by DAF sequencing.The expression of CD55 on patient′s cell membrane was deficient.Conclusion This high frequency antibody was identified as anti-IFC.The transient depression in CD55 protein may due to the patient′s GI system abnormalities.

关 键 词：Cromer血型系统 抗-IFC 酶处理谱细胞 CD55蛋白 DAF 

分 类 号：R457.11[医药卫生—治疗学]