基于血站实验室质量指标评价的抗-HCV检测分析  

Anti-HCV detection based on laboratory quality index evaluation of blood station

作　　者：秦龑丽 吴康乐[1] 戴田[1] 冯惟萍[1] 杨树龙[1] QIN Yanli;WU Kangle;DAI Tian;FENG Weiping;YANG Shulong(Blood Center of Gansu Province,Lanzhou 130046,China)

机构地区：[1]甘肃省红十字血液中心,甘肃兰州730046

出　　处：《中国输血杂志》2021年第7期770-772,共3页Chinese Journal of Blood Transfusion

摘　　要：目的评价本实验室抗-HCV检测能力,探讨可能影响抗-HCV检测的因素,为评估实验室能力提供数据和依据。方法统计2019-2020年抗-HCV需进行重复检测标本数量(IR)、重复检测为反应性标本数量(RR)和试剂使用率等指标,与全国同组试剂比较分析。结果抗-HCV检测不合格率平均值为0.25%(最低为0.19%,33/17 774,最高为0.37%,44/11 940);试剂1与试剂2的复检率差异有统计学意义(P<0.05);试剂1与试剂2的复检符合率经过比较差异无统计学意义(P>0.05);试剂1与试剂2的复检符合率均呈现缓慢上升趋势;试剂1与试剂2的单试剂不合格率差异有统计学意义(P<0.05);试剂使用率与全国同组试剂的平均水平基本持平。结论本实验室的抗-HCV检测指标相对稳定,但人员培训、设备性能、环境等其他因素也会对实验室的检测情况产生影响,可推行各要素的精细化管理,利用血站血液检测实验室质量指标的室间质评回报报告,加强分析,持续改进,进一步提高本实验室的抗-HCV检测能力。Objective To evaluate the anti-HCV detection ability of our laboratory,and explore the factors that may affect anti-HCV detection,so as to provide data and basis for the evaluation of laboratory ability.Methods The number of initial reactive(IR)and repeated reactive(RR)samples and the reagent utilization rate in anti-HCV from 2019 to 2020 were compared with the national reagents of the same group.Results 1)The average unqualified rate of anti-HCV detection was 0.25%,with the lowest rate at 0.19%,33/17774,and the highest rate at 0.37%,44/11940;2)The retest rates of reagent 1 and reagent 2 were significantly different(P<0.05);3)The RR/IR rates of reagent 1 and reagent 2 showed no significant difference(P>0.05),while the RR/IR rates of reagent 1 and reagent 2 showed a slow upward trend;4)The solo reagent unqualified rate of reagent 1 and reagent 2 showed statistically significant difference(P<0.05);5)The reagent utilization rate was basically the same as the national average level of reagents in the same group.Conclusion The anti-HCV detection indicators of our laboratory are relatively stable,but other factors such as personnel training,equipment performance and environment also have an impact on the detection ability of laboratories.Fine management of various element should be carried out,and external quality assessment reports of blood testing laboratory should be analyzed to further improve the anti-HCV detection ability of the laboratory.

关 键 词：血站实验室 抗-HCV ELISA 质量指标 

分 类 号：R457.12[医药卫生—治疗学] R512.62[医药卫生—临床医学]