玉米幼胚抗草甘膦基因的遗传转化研究  

Genetic Transformation of Glyphosate-tolerant Genes in Maize Immature Embryos

作　　者：刘悦 王立达[1] 兰英 李青超[1] 赵秀梅[1] 杨莹[1] 周传余[1] LIU Yue;WANG Li-da;LAN Ying;LI Qing-chao;ZHAO Xiu-mei;YANG Ying;ZHOU Chuan-yu(Qiqihar Branch,Heilongjiang Academy of Agricultural Sciences,Qiqihar 161006,China)

机构地区：[1]黑龙江省农业科学院齐齐哈尔分院,黑龙江齐齐哈尔161006

出　　处：《黑龙江农业科学》2021年第8期38-41,47,共5页Heilongjiang Agricultural Sciences

基　　金：黑龙江省农业科学院“农业科技创新跨越工程”专项(HNK2019CX14)。

摘　　要：为培育优质高产的玉米植株,本研究利用农杆菌介导法,对Hi-Ⅱ玉米幼胚抗草甘膦基因的遗传转化进行研究,将抗草甘膦基因Epsps转入Hi-Ⅱ玉米幼胚,通过大量幼胚转化、共培养及其分化筛选,获得具有抗草甘膦抗性的玉米植株。计算转化率,研究外源基因在后代的遗传表达及抗性情况,并分析在农杆菌介导玉米幼胚的遗传转化中影响转化效率的主要因素。结果表明:玉米幼胚大小在1.5~1.8 mm时侵染效果最好,并成功获得了9株具有草甘膦抗性的阳性转化植株,经田间初测所获得的转基因植株具有草甘膦抗性。In this study,Agrobacterium tumefaciens-mediated method was used to study the genetic transformation of glyphosate-tolerant genes in Hi-Ⅱmaize.Immature glyphosate gene Epsps was transferred into Hi-Ⅱmaize immature embryos,co-culture and differentiation screening,to obtain resistance to glyphosate resistance of maize plants.The conversion rate was calculated,the genetic expression and resistance of exogenous genes in offspring were studied,and the main factors influencing the transformation efficiency were analyzed in the genetic transformation of Agrobacterium tumefaciens-mediated immature embryos.The results showed that at the 1.5-1.8 mm maize embryo size,the infection effect was the best,and 9 plants with glyphosate resistance were successfully transformed,glyphosate resistance of transgenic plants obtained by inter-field testing.The results of this study can provide scientific basis for the study of high quality cultivation of transgenic maize.

关 键 词：农杆菌介导 Hi-Ⅱ玉米 抗草甘膦基因 转化 

分 类 号：S51[农业科学—作物学]